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Positioning of sperm tail longitudinal columns depend on Nsun7, an RNA binding protein destabilizing elongated spermatid transcripts (RNA-Seq)

GSE281586 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/04/23 Platform GPL24247
Summary
Spermatozoid’s flagella assemble in transcriptionally silent spermatids and thus depends on post-transcriptional regulation of gene expression. Mutations in Nsun7 gene are known to cause male infertility in human and mice. We identified m5C-specific Nsun7 RNA methyltransferase as a protein present in elongated spermatids and interacting with RNAs specific for this type of spermatozoid’s precursor cells. Inactivation of Nsun7 gene in mice leads to upregulation of its RNA interactors, thus indicating that Nsun7 downregulates a set of RNAs in the elongated spermatids. A physiologic consequence of Nsun7 gene knockout is male infertility, which is mechanistically explained by the observed mispositioning of longitudinal columns relative to the axonemal microtubular doublets leading to motility defect.
Published in
Positioning of sperm tail longitudinal columns depends on NSUN7, an RNA-binding protein destabilizing elongated spermatid transcripts
Guseva EA, Averina OA, Isaev SV et al. · RNA (New York, N.Y.) 2025 · PMID 40032361 · doi:10.1261/rna.080320.124
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Also filed as BioProject PRJNA1184756 and SRA study SRP544488. Searching any of these in the dataset finder brings you back here.

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