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Expression profiling of purified cells after (mature) paneth cell depletion.

GSE281800 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2025/02/26 Platform GPL24247
Summary
We used transgenic mice expressing diphtheria toxin in a paneth cell specific manner to eablate paneth cells, but found that we could still detect and also purify (paneth) cells in the transgenic mice. We purified this population and a control WT population of paneth cells for expression profiling, with the goal to check if the diphtheria toxin expression system had low penetrance or if our PC population in TG mice was immature , and thus did not yet express the active diphtheria toxin.
Published in
Detection of chimeric alpha-defensin transcripts and peptides in mouse Paneth cells
Timmermans S, Wallaeys C, De Beul S et al. · Frontiers in immunology 2025 · PMID 39981239 · doi:10.3389/fimmu.2025.1543059
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Also filed as BioProject PRJNA1185731 and SRA study SRP545000. Searching any of these in the dataset finder brings you back here.

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