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IL-2Rα is Dispensable for Murine B Cell Development and Humoral Response

GSE282135 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2025/05/07 Platform GPL24247
Summary
The cytokine IL-2 plays a pivotal role in the immune system, specifically in the proliferation of T, B, and NK cells. The alpha subunit of the IL-2 receptor, IL-2Rα (CD25), is known to regulate the expansion and differentiation of T lymphocytes. CD25 is also expressed in developing B cells; however, its B cell intrinsic role remains undefined. We generated a mouse model with a B cell-specific deletion of CD25 to ascertain its role in B cell development and function. Unexpectedly, we found that the loss of CD25 had no impact on B cell development, homeostasis, or immune response to model antigens. Additionally, while CD25 expression was upregulated in activated splenic B cells, its absence did not affect class switch recombination in vitro or in vivo. We conclude that in contrast to its critical role in T cell differentiation and function, and despite its expression in developing and activated B cells, CD25 does not have any significant role in B cell development and adaptive immune functions.
Published in
IL-2Rα is dispensable for murine B cell development and humoral response
Chowdhury P, Belcheva KT, Smolkin RM et al. · Journal of immunology (Baltimore, Md. : 1950) 2025 · PMID 40096628 · doi:10.1093/jimmun/vkae045
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Direct links to NCBI, no account and no request form: the whole study as GSE282135_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1187268 and SRA study SRP545738. Searching any of these in the dataset finder brings you back here.

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