← BioTransfer GEO Dataset Finder
GEO series

Acetylcholine derived from B lymphocytes controls liver regeneration

GSE283252 Mus musculus (mouse) RNA-seq (gene expression) 26 samples Submitted 2025/05/02 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 26 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
26 samples: 7 × ChAT-GFP negative liver B cells, sham surgery, vs 7 × ChAT-GFP positive liver B cells, 4 hours post-PHX, vs 6 × ChAT-GFP negative liver B cells, 4 hours post-PHX, vs 6 × ChAT-GFP positive liver B cells, sham surgery,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
B cell-derived acetylcholine promotes liver regeneration by regulating Kupffer cell and hepatic CD8(+) T cell functionImmunity · 2025
Summary (written by the submitters)
Humans recover from hepatic injury largely by liver regeneration. This complicated process requires hepatocyte proliferation driven by collaboration amongst multiple hepatic cell types. Immune cells also contribute, with some producing mitogenic cytokines such as IL-6 but others damaging hepatocytes by secreting IFNγ. A better understanding of immune cell functions in liver regeneration is necessary to improve clinical interventions.
Read the full summary ↓
Here, we demonstrate that B cells expressing choline acetyltransferase (ChAT), the enzyme synthesizing acetylcholine (ACh), are specifically required for liver regeneration. Mice lacking ChAT+ B cells subjected to partial hepatectomy (PHX) display greater mortality due to failed liver regeneration. We show that both Kupffer cells and hepatic CD8+ T cells express the α7 nicotinic ACh receptor (nAChR; encoded by Chrna7), and that liver regeneration is also disrupted in mice lacking α7 nAChR. Thus, two regulatory axes promoting hepatocyte proliferation operate following PHX: one where hepatic ChAT+ B cells and α7 nAChR+ Kupffer cells interact to drive IL-6 production by Kupffer cells, and another where ChAT+ B cells and α7 nAChR+ hepatic CD8+ T cells interact to reduce IFNγ production by CD8+ T cells. Our work offers novel insights into liver regeneration mechanisms that may point to new therapies for liver damage.
Published in
B cell-derived acetylcholine promotes liver regeneration by regulating Kupffer cell and hepatic CD8(+) T cell function
Modares NF, Hendrikse LD, Smith LK et al. · Immunity 2025 · PMID 40286791 · doi:10.1016/j.immuni.2025.04.002
This dataset
Open GSE283252 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE283252_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1192963 and SRA study SRP548629. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40286791) ↗
Study design
7 × ChAT-GFP negative liver B cells, sham surgery, vs 7 × ChAT-GFP positive liver B cells, 4 hours post-PHX, vs 6 × ChAT-GFP negative liver B cells, 4 hours post-PHX, vs 6 × ChAT-GFP positive liver B cells, sham surgery,

Supports a between-group comparison across 26 samples.

How we read this: 4 replicated groups read from 26 sample titles; they account for 26 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.