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RNA Sequencing of Mycobacterium tuberculosis-infected macrophages treated with meclizine.

GSE283874 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/08/14 Platform GPL24247
Summary
The ability of Mycobacterium tuberculosis (Mtb) to tolerate antibiotics is a major impediment in the treatment of tuberculosis. This antibiotic tolerance is influenced by the host cells in which the bacteria reside, as Mtb senses host cues, and the bacterial response can provide an enhanced ability to withstand anti-TB drugs. Previouslyt, we have shown that Mtb exhibits redox heterogeneity in terms of its mycothiol redox potential (EMSH) giving rise to three broad subpopulations EMSH-reduced, EMSH-basal, and EMSH-oxidized Mtb specifically inside the macrophages. Among these, the EMSH-reduced Mtb exhibit enahnced tolerance to several anti-TB drugs while the EMSH-basal and EMSH-oxidized subpopulations are more susceptible. In this study, we have discovered a novel host-directed therapy molecule- meclizine, which enhances the drug susceptible population and diminishes the tolerant population.
Published in
Bioenergetic reprogramming of macrophages reduces drug tolerance in Mycobacterium tuberculosis
Yadav V, Sahoo S, Malhotra N et al. · Nature communications 2025 · PMID 41130947 · doi:10.1038/s41467-025-64407-w
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Also filed as BioProject PRJNA1195948 and SRA study SRP550308. Searching any of these in the dataset finder brings you back here.

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