Feeder-free culture of naive human PSCs preserving embryonic, extraembryonic and blastoid potential
RNA-seq (gene expression) · human · 57 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 57 samples: 28 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Serum coating enables feeder-free culture of naive human pluripotent stem cells preserving developmental potentialThe EMBO journal · 2026
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE284370_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1198893 and SRA study SRP551747. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 56 samples.
How we read this: 28 replicated groups read from 57 sample titles; they account for 56 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8682886 HPD06 on MEFs, RNA-Seq, Replicate 1 HPD06 on MEFs, RNA-Seq,
- GSM8682887 HPD06 on MEFs, RNA-Seq, Replicate 2 HPD06 on MEFs, RNA-Seq,
- GSM8682888 HPD06_on serum, RNA-Seq, Replicate 1 HPD06_on serum, RNA-Seq,
- GSM8682889 HPD06_on serum, RNA-Seq, Replicate 2 HPD06_on serum, RNA-Seq,
- GSM8682890 HPD03 on MEFs, RNA-Seq, Replicate 1 HPD03 on MEFs, RNA-Seq,
- GSM8682891 HPD03 on MEFs, RNA-Seq, Replicate 2 HPD03 on MEFs, RNA-Seq,
- GSM8682892 HPD03 on serum, RNA-Seq, Replicate 1 HPD03 on serum, RNA-Seq,
- GSM8682893 HPD03 on serum, RNA-Seq, Replicate 2 HPD03 on serum, RNA-Seq,
- GSM8682894 Shef6 on serum, RNA-Seq, Replicate 1 Shef6 on serum, RNA-Seq,
- GSM8682895 Shef6 on serum, RNA-Seq, Replicate 2 Shef6 on serum, RNA-Seq,
- GSM8682896 HPD06 on MEFs, Exome-Seq, Replicate 1 HPD06 on MEFs, Exome-Se…
- GSM8682897 HPD06 on MEFs, Exome-Seq, Replicate 2 HPD06 on MEFs, Exome-Se…
- GSM8682898 HPD06 on serum, Exome-Seq, Replicate 1 HPD06 on serum, Exome-S…
- GSM8682899 HPD06 on serum, Exome-Seq, Replicate 2 HPD06 on serum, Exome-S…
- GSM8682900 HPD03 on MEFs, Exome-Seq, Replicate 1 HPD03 on MEFs, Exome-Se…
- GSM8682901 HPD03 on MEFs, Exome-Seq, Replicate 2 HPD03 on MEFs, Exome-Se…
- GSM8682902 HPD03 on serum, Exome-Seq, Replicate 1 HPD03 on serum, Exome-S…
- GSM8682903 HPD03 on serum, Exome-Seq, Replicate 2 HPD03 on serum, Exome-S…
- GSM8686198 blastoids, feeder-free
- GSM8741212 Naive serum-adapted Shef6 on serum, PXGL d0, RNA-Seq, Replicate 1 Naive serum-adapted She…
- GSM8741213 Naive serum-adapted Shef6 on serum, PXGL d0, RNA-Seq, Replicate 2 Naive serum-adapted She…
- GSM8741214 Naive serum-adapted Shef6 on Geltrex, PXGL d0, RNA-Seq, Replicate 1 Naive serum-adapted She…
- GSM8741215 Naive serum-adapted Shef6 on Geltrex, PXGL d0, RNA-Seq, Replicate 2 Naive serum-adapted She…
- GSM8741216 Naive serum-adapted Shef6 on Geltrex, N2B27 d2, RNA-Seq, Replicate 1 Naive serum-adapted She…
- GSM8741217 Naive serum-adapted Shef6 on Geltrex, N2B27 d2, RNA-Seq, Replicate 2 Naive serum-adapted She…
- GSM8741218 Naive serum-adapted Shef6 on Geltrex, N2B27 d3, RNA-Seq, Replicate 1 Naive serum-adapted She…
- GSM8741219 Naive serum-adapted Shef6 on Geltrex, N2B27 d3, RNA-Seq, Replicate 2 Naive serum-adapted She…
- GSM8741220 Naive serum-adapted Shef6 on Geltrex, N2B27 d4, RNA-Seq, Replicate 1 Naive serum-adapted She…
- GSM8741221 Naive serum-adapted Shef6 on Geltrex, N2B27 d4, RNA-Seq, Replicate 2 Naive serum-adapted She…
- GSM8741222 Naive serum-adapted Shef6 on Geltrex, N2B27 d6, RNA-Seq, Replicate 1 Naive serum-adapted She…
- GSM8741223 Naive serum-adapted Shef6 on Geltrex, N2B27 d6, RNA-Seq, Replicate 2 Naive serum-adapted She…
- GSM8741224 Naive serum-adapted Shef6 on Geltrex, N2B27 d8, RNA-Seq, Replicate 1 Naive serum-adapted She…
- GSM8741225 Naive serum-adapted Shef6 on Geltrex, N2B27 d8, RNA-Seq, Replicate 2 Naive serum-adapted She…
- GSM8741226 Naive serum-adapted Shef6 on Geltrex, E8 d2, RNA-Seq, Replicate 1 Naive serum-adapted She…
- GSM8741227 Naive serum-adapted Shef6 on Geltrex, E8 d2, RNA-Seq, Replicate 2 Naive serum-adapted She…
- GSM8741228 Naive serum-adapted Shef6 on Geltrex, E8 d3, RNA-Seq, Replicate 1 Naive serum-adapted She…
- GSM8741229 Naive serum-adapted Shef6 on Geltrex, E8 d3, RNA-Seq, Replicate 2 Naive serum-adapted She…
- GSM8741230 Naive serum-adapted Shef6 on Geltrex, E8 d4, RNA-Seq, Replicate 1 Naive serum-adapted She…
- GSM8741231 Naive serum-adapted Shef6 on Geltrex, E8 d4, RNA-Seq, Replicate 2 Naive serum-adapted She…
- GSM8741232 Naive serum-adapted Shef6 on Geltrex, E8 d6, RNA-Seq, Replicate 1 Naive serum-adapted She…
+ 17 more — browse all 57 samples with per-sample file links →
- GSE287199 Feeder-free culture of naive human PSCs preserving embryonic, extraembryonic and blastoid potential 38 samples shares extraembryonic, feeder-free, preserving, embryonic, potential, blastoid, culture, naive
- GSE284368 Feeder-free culture of naive human pluripotent stem cells retaining embryonic, extraembryonic and blastoid generation potential [RNA-Seq] 10 samples shares extraembryonic, feeder-free, embryonic, potential, blastoid, culture, naive
- GSE252316 Novel culture systems for naive human embryonic stem cells based on chemical molecule screening 26 samples shares embryonic, culture, naive
- GSE252312 Novel culture systems for naive human embryonic stem cells based on chemical molecule screening [RNA-seq] 16 samples shares embryonic, culture, naive
- GSE85066 In vitro cultured H7 human embryonic stem cells (WiCell) and H7-derived downstream early mesoderm progenitors 12 samples shares embryonic, culture
- GSE247634 α-Ketoglutarate promotes trophectoderm induction and maturation from naive human embryonic stem cells 28 samples shares embryonic, naive
- GSE247631 α-Ketoglutarate promotes trophectoderm induction and maturation from naive human embryonic stem cells [RNA-Seq] 16 samples shares embryonic, naive
- GSE271234 Generation of self-renewing neuromesodermal progenitors with neuronal and skeletal muscle bipotential from human embryonic stem cells 15 samples shares embryonic, potential
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.