GEO series

scRNAseq of stretch-mediated skin expansion in mouse

GSE285079 Mus musculus (mouse) Single-cell RNA-seq 18 samples Submitted 2026/05/14 Platform GPL30172

At a glance

Single-cell RNA-seq · mouse · 18 samples

What it is: Single-cell RNA-seqGene activity measured in thousands of individual cells, so cell types can be told apart.
Organism: Mus musculus (mouse)
Samples: 18 samples: 18 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: A stretch-responsive fibroblast program promotes epidermal stem cell self-renewal during skin expansionNature communications · 2026

Summary (written by the submitters)

Using an in vivo mouse model of stretch-mediated skin expansion, we have performed scRNAseq (10x Genomics) on control (CTRL) skin and expanded (EXP) skin at 3 time points: day 1, day 2 and day 4. Stretch-mediated expansion is used in reconstructive and cosmetic surgeries to produce extra skin, however the mechanisms behind it are not well understood. This study represents the first full skin scRNAseq study of in vivo stretch-mediated skin expansion.

Published in

A stretch-responsive fibroblast program promotes epidermal stem cell self-renewal during skin expansion

Aguilera Stewart C, Alar C, Gozza GA et al. · Nature communications 2026 · PMID 42285958 · doi:10.1038/s41467-026-73979-0

This dataset

Open GSE285079 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE285079_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Single-cell studies usually ship their counts as 10x files (matrix.mtx, barcodes.tsv, features.tsv), an .h5 file, or an .rds / .h5ad object that Seurat or Scanpy can open. Look for those among the supplementary files.

Also filed as BioProject PRJNA1201155 and SRA study SRP553009. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42285958) ↗

Study design

18 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 18 sample titles: 18 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8696180 Day 1 (Sequencing round 1) CTRL skin
GSM8696181 Day 1 (Sequencing round 1) EXP skin
GSM8696182 Day 1 (Sequencing round 2) CTRL Hashing
GSM8696183 Day 1 (Sequencing round 2) CTRL skin
GSM8696184 Day 1 (Sequencing round 2) EXP skin
GSM8696185 Day 1 (Sequencing round 2) EXP skin Hashing
GSM8696186 Day 2 (Sequencing round 1) CTRL skin
GSM8696187 Day 2 (Sequencing round 1) CTRL skin Hashing
GSM8696188 Day 2 (Sequencing round 1) EXP skin
GSM8696189 Day 2 (Sequencing round 1) EXP skin Hashing
GSM8696190 Day 2 (Sequencing round 2) CTRL skin
GSM8696191 Day 2 (Sequencing round 2) CTRL skin Hashing
GSM8696192 Day 2 (Sequencing round 2) EXP skin
GSM8696193 Day 2 (Sequencing round 2) EXP skin Hashing
GSM8696194 Day 4 (Sequencing round 1) CTRL skin
GSM8696195 Day 4 (Sequencing round 1) CTRL skin Hashing
GSM8696196 Day 4 (Sequencing round 1) EXP skin
GSM8696197 Day 4 (Sequencing round 1) EXP skin Hashing

Datasets on a similar topic

GSE284808 Bulk RNAseq of sorted fibroblasts from a mouse model of stretch-mediated skin expansion 22 samples shares stretch-mediated, expansion, skin
GSE213104 Gene expression profile at single cell level of Merkel cells from embryonic, neonatal and postnatal mouse skin 1057 samples shares skin
GSE245747 Transcriptome Analysis in Mouse Skin After Exposure to Ultraviolet Radiation from a Canopy Sunbed 145 samples shares skin
GSE265909 yHSC and oHSC scRNAseq with oHSC AThi ATlo Index Sorting. Assessing the transcriptional changes associated with autophagy engagement in old hematopoietic stem cells at single cell resolution. [scRNA-Seq] 563 samples shares scrnaseq
GSE328310 Rapid evolution of mouse saliva through regulatory rewiring and gene expansions 60 samples shares expansion
GSE310560 Deep profiling of multiple mouse tumor microenvironments using scRNAseq 56 samples shares scrnaseq
GSE279468 Gene expression profile at single cell level of Braf-mutant mouse melanoma tumor and skin 53 samples shares skin
GSE280510 scRNAseq and CITEseq of aged HSPCs derived from FlkSwitch mouse model 24 samples shares scrnaseq

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.