GEO series

ACBP in brown adipose tissue acts as a negative regulator of adaptive thermogenesis

GSE285403 Mus musculus (mouse) RNA-seq (gene expression) 17 samples Submitted 2025/05/08 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 17 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 17 samples: 5 × HFD_WT vs 5 × HFD_KO vs 4 × Chow_WT vs 3 × Chow_KOGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Acyl CoA-binding protein in brown adipose tissue acts as a negative regulator of adaptive thermogenesisMolecular metabolism · 2025

Summary (written by the submitters)

The defective activity of brown adipose tissue (BAT) is linked to obesity and cardiometabolic diseases. While there is extensive knowledge about the biological signals that trigger BAT thermogenesis, information regarding active repressors that may contribute to the pathological impairment of BAT function is quite limited. Acyl CoA-Binding Protein (ACBP), also known as Diazepam Binding Inhibitor (DBI), is a protein with intracellular functions related to lipid metabolism.

Read the full summary ↓

It can also be secreted and act as a circulating regulatory factor that affects multiple tissues and organs. We discovered that ACBP expression and release in BAT are suppressed by noradrenergic, cAMP-dependent signals that stimulate thermogenesis. This regulation occurs through mechanisms involving gene expression and autophagy-related processes. Mice with targeted ablation of the Acbp gene in brown adipocytes exhibit enhanced BAT thermogenic activity and protection against obesity and glucose intolerance induced by a high-fat diet. This is associated with a remodeling of the BAT transcriptome, characterized by the induction of genes related to BAT thermogenesis. Treatment of brown adipocytes with exogenous ACBP suppressed oxidative activity, lipolysis, and the expression of genes associated with thermogenesis. ACBP inhibits the noradrenergic-induced phosphorylation of p38 MAP kinase and CREB, major intracellular mediators of brown adipocyte thermogenesis. Our findings identify the ACBP system as a crucial auto-regulatory repressor of BAT thermogenesis, which responds reciprocally to the noradrenergic induction of BAT activity.

Published in

Acyl CoA-binding protein in brown adipose tissue acts as a negative regulator of adaptive thermogenesis

Blasco-Roset A, Quesada-López T, Mestres-Arenas A et al. · Molecular metabolism 2025 · PMID 40220929 · doi:10.1016/j.molmet.2025.102153

This dataset

Open GSE285403 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE285403_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1203291 and SRA study SRP553969. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40220929) ↗

Study design

5 × HFD_WT vs 5 × HFD_KO vs 4 × Chow_WT vs 3 × Chow_KO

Supports a between-group comparison across 17 samples.

How we read this: 4 replicated groups read from 17 sample titles; they account for 17 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8701755 Chow_WT_1 Chow_WT
GSM8701756 Chow_WT_2 Chow_WT
GSM8701757 Chow_WT_3 Chow_WT
GSM8701758 Chow_WT_4 Chow_WT
GSM8701759 Chow_KO_2 Chow_KO
GSM8701760 Chow_KO_3 Chow_KO
GSM8701761 Chow_KO_4 Chow_KO
GSM8701762 HFD_WT_1 HFD_WT
GSM8701763 HFD_WT_2 HFD_WT
GSM8701764 HFD_WT_3 HFD_WT
GSM8701765 HFD_WT_4 HFD_WT
GSM8701766 HFD_WT_5 HFD_WT
GSM8701767 HFD_KO_1 HFD_KO
GSM8701768 HFD_KO_2 HFD_KO
GSM8701769 HFD_KO_3 HFD_KO
GSM8701770 HFD_KO_4 HFD_KO
GSM8701771 HFD_KO_5 HFD_KO

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.