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Single-cell transcriptional profiling and gene regulatory network modeling in Tg2576 mice reveal gender-dependent molecular features preceding Alzheimer-like pathologies

GSE285694 Mus musculus Expression profiling by high throughput sequencing; Other 4 samples Submitted 2025/01/02 Platform GPL19057
Summary
To identify disease-related molecular processes that occur before the onset of detectable neuropathology in Alzheimer's disease (AD), we performed a comprehensive single-cell analysis of disease-related molecular sex differences in transcriptomic data from the neocortex of 24-week-old Tg2576 mice and wild-type littermate controls. Cell type-specific changes were investigated at the level of individual genes, pathways and gene regulatory networks. The analyses revealed significant cell-type-specific gene expression changes in individual genes, pathways and sub-networks, including sex-specific and sex-dimorphic changes in both upstream transcription factors and their downstream targets, before the onset of overt disease. The study opens a window into the molecular events that may determine sex-specific susceptibility to Alzheimer's disease and uncovers tractable target candidates for potential sex-specific precision medicine for AD.
Published in
Single-Cell Transcriptional Profiling and Gene Regulatory Network Modeling in Tg2576 Mice Reveal Gender-Dependent Molecular Features Preceding Alzheimer-Like Pathologies
Ali M, Huarte OU, Heurtaux T et al. · Molecular neurobiology 2024 · PMID 35980567 · doi:10.1007/s12035-022-02985-2
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Direct links to NCBI, no account and no request form: the whole study as GSE285694_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1205664 and SRA study SRP554904. Searching any of these in the dataset finder brings you back here.

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