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scRNA-seq of lung cells from murine model of ozone induced asthma exacerbation

GSE285723 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2025/12/02 Platform GPL24247
Summary
Ozone (O3) inhalation triggers asthmatic airway hyperresponsiveness (AHR), but the mechanisms by which this occurs are unknown. Previously, we developed a murine model of dust mite, ragweed, and aspergillus (DRA)-induced allergic lung inflammation followed by O3 exposure for mechanistic investigation. In this model, mice exposed to DRA or O3 alone do not develop AHR, but the two exposures combined have a synergistic effect and the DRA+O3 exposure group develops significant AHR. The present study used single cell RNA-sequencing for unbiased profiling of cells within the lungs of mice exposed to DRA, O3, or DRA+O3, to identify the components that contribute to AHR. Inflammation is generally considered the underlying cause of AHR, so immune cells were of specific focus.
Published in
Single-Cell RNA Sequencing Identifies a Unique Macrophage Population in a Mouse Model of Ozone-induced Asthma Exacerbation
Ray JL, Walum J, Jelic D et al. · American journal of respiratory cell and molecular biology 2025 · PMID 40106801 · doi:10.1165/rcmb.2024-0358OC
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Also filed as BioProject PRJNA1205713 and SRA study SRP554983. Searching any of these in the dataset finder brings you back here.

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