GEO series

The conserved N-terminal SANT1-binding domain (SBD) of EZH2 Regulates PRC2 Activity [delSBD-ChIP-Rx]

GSE286459 Mus musculus (mouse) ChIP-seq / ATAC-seq (genome binding and open chromatin) 18 samples Submitted 2025/01/13 Platform GPL19057

At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 18 samples

What it is: ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism: Mus musculus (mouse)
Samples: 18 samples: 18 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: The conserved N-terminal SANT1-binding domain (SBD) of EZH2 regulates PRC2 activityGenes & development · 2026

Summary (written by the submitters)

Polycomb group proteins maintain gene expression patterns established during early development, with Polycomb Repressive Complex 2 (PRC2) methyltransferase a key regulator of cell differentiation, identity and plasticity. Consequently, extensive somatic mutations in PRC2, including gain- or loss- of function (GOF or LOF), are observed in human cancers.

Read the full summary ↓

The regulation of chromatin structure by PRC2 is critically dependent on its EZH2 (Enhancer of Zeste Homolog 2) subunit, which catalyzes the methylation of histone H3 lysine 27 (H3K27). Recent structural studies of PRC2 revealed extensive conformational changes in the non-catalytic EZH2 N-terminal SANT-Binding Domain (SBD) during PRC2 activation, though the functional significance remains unclear. Here, we investigate how the SBD regulates PRC2 function. The domain is highly conserved in metazoans, dispensable for PRC2 assembly and chromatin localization, yet required for genome-wide histone H3K27 methylation. Further, we show that an intact SBD is necessary for the proliferation of EZH2-addicted lymphomas, and its deletion in the presence of EZH2 GOF mutations inhibits cancer cell growth. These observations provide new insights to the regulation of PRC2 activity in normal development and malignancy.

Published in

The conserved N-terminal SANT1-binding domain (SBD) of EZH2 regulates PRC2 activity

Patriotis AL, Barrows DW, Soto-Feliciano Y et al. · Genes & development 2026 · PMID 41663271 · doi:10.1101/gad.353070.125

This dataset

Open GSE286459 in finder → Find more mouse ChIP / ATAC / CUT&Tag datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE286459_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1209536 and SRA study SRP556731. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41663271) ↗

Study design

18 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 18 sample titles: 18 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8728091 A1-input-ChIP, mESC
GSM8728092 A1-JEZH2-ChIP, mESC
GSM8728093 A1-SUZ12-ChIP, mESC
GSM8728094 A1-H3K27me3-ChIP, mESC
GSM8728095 A1-H3K27me32ChIP, mESC
GSM8728096 EZH2-KO-input-ChIP, mESC
GSM8728097 EZH2-KO-EZH2-ChIP, mESC
GSM8728098 EZH2-KO-SUZ12-ChIP, mESC
GSM8728099 EZH2-KO-H3K27me3-ChIP, mESC
GSM8728100 EZH2-KO-H3K27me32ChIP, mESC
GSM8728101 EZH2-ΔSBD-input-ChIP, mESC
GSM8728102 EZH2-ΔSBD-EZH2-ChIP, mESC
GSM8728103 EZH2-ΔSBD-SUZ12-ChIP, mESC
GSM8728104 EZH2-ΔSBD-H3K27me3-ChIP, mESC
GSM8728105 EZH2-ΔSBD-H3K27me2-ChIP, mESC
GSM9305630 A1-H3K27ac-ChIP-mESC
GSM9305631 EZH2-H3K27ac-ChIP-mESC
GSM9305632 EZH2-ΔSBD-H3K27ac-ChIP-mESC

Datasets on a similar topic

GSE286457 The conserved N-terminal SANT1-binding domain (SBD) of EZH2 Regulates PRC2 Activity [MTF2-JARID2] 15 samples shares ezh2, prc2, sbd, sant1-binding, n-terminal, conserved, activity
GSE291366 A conserved function of EZH2-PRC2 in the repression of primitive endoderm cell fate [CUT&Tag] 12 samples shares ezh2, prc2, conserved
GSE291454 A conserved function of EZH2-PRC2 in the repression of primitive endoderm cell fate [RNA-Seq - mouse] 22 samples shares ezh2, prc2, conserved
GSE239636 Cancer-associated Histone H3 N-terminal arginine mutations disrupt PRC2 activity and impair differentiation [CUT&RUN] 60 samples shares prc2, n-terminal, activity
GSE239632 Cancer-associated Histone H3 N-terminal arginine mutations disrupt PRC2 activity and impair differentiation [RNA-Seq] 15 samples shares prc2, n-terminal, activity
GSE302605 Functional Comparison to Ezh2 Reveals PRC2-Independent Functions of Jarid2 in Hematopoietic Stem Cell Lineage Commitment 23 samples shares ezh2, prc2
GSE306783 EPOP and MTF2 Activate PRC2 Activity through DNA-sequence specificity 34 samples shares prc2, activity
GSE257545 Top2a Differentially Modulates Promoter Activity in Embryonic Stem Cells via Trim28 Recruitment and PRC2 Displacement [ChIP-Seq] 16 samples shares prc2, activity

Search all mouse ChIP / ATAC / CUT&Tag datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.