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Retinoic acid signaling activation in early intermediate mesoderm differentiation promotes dual lineage of intermediate mesoderm specification and enhances full-segment kidney organoid generation

GSE287170 Homo sapiens (human) RNA-seq (gene expression) 2 samples Submitted 2025/12/17 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 2 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
2 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Induction of Nephron-Ductal Dual Lineages via Early Retinoic Acid Signaling Establishes a Platform for Fully Patterned Kidney OrganoidsAdvanced healthcare materials · 2025
Summary (written by the submitters)
During kidney development, an intermediate mesoderm (IM) give rise to distinct structures: the ureteric bud (UB) and the metanephric mesenchyme (MM). These structures differentiate further into the collecting duct and nephron, forming a mature kidney. Generating functional kidney organoids has been challenging due to incomplete development of the UB.
Read the full summary ↓
Researchers have overcome this limitation by differentiating UB and MM separately and co-culturing them to generate kidney organoids. However, this study developed a co-culture-free method using retinoic acid (RA), plays important role in the anterior IM differentiation and BMP7 secreted by UB in vivo development. This protocol provided not only simplifies the complexity of kidney organoid generation but also advances our understanding of the crucial signaling pathways involved in kidney development.
Published in
Induction of Nephron-Ductal Dual Lineages via Early Retinoic Acid Signaling Establishes a Platform for Fully Patterned Kidney Organoids
Lim JH, Kim S, Lee DH et al. · Advanced healthcare materials 2025 · PMID 40906456 · doi:10.1002/adhm.202502044
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE287170_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1211540 and SRA study SRP557826. Searching any of these in the dataset finder brings you back here.

Samples in this study

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