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Transcriptional profile of B16-OVA cells with PUM1 knockout

GSE287952 Mus musculus Expression profiling by high throughput sequencing 9 samples Submitted 2025/10/22 Platform GPL28330
Summary
PUM1 is a highly conserved RNA-binding protein that plays a key role in regulating mRNA stability. B16-OVA knockout PUM1 cell lines were constructed using CRISPR/CAS9 gene editing technology, and it was found that the PUM1 knockout reduce cell proliferation through indcuing G2/M phase arrest and causing cell apoptosis. From RNA-seq sequencing results, GO enrichment analysis showed that differential expression genes were mainly enriched in the negative regulation of viral process, response to interferon-beta, defense response to virus, defense response to symbionts and other pathways closely related to immunity. KEGG enrichment analysis showed that differential genes were mainly enriched in JAK-STAT signaling pathway, TNF signaling pathway, NF-kappa B signaling pathway, PI3K-Akt signaling pathway, MAPK signaling pathway and NOD-like receptor signaling pathway.
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Direct links to NCBI, no account and no request form: the whole study as GSE287952_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1215352 and SRA study SRP559688. Searching any of these in the dataset finder brings you back here.

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