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Neuronal type-specific modulation of cognition and AP-1 signaling by early-life rearing conditions. [RNA-Seq]

GSE288334 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2025/09/23 Platform GPL17021
At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
18 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Neuronal type-specific modulation of cognition and AP-1 signaling by early-life rearing conditionsNature communications · 2025
Summary (written by the submitters)
Environmental conditions profoundly influence cognitive development, particularly during early life. Transcriptional and epigenetic mechanisms may serve as molecular substrates for the lasting effects of environmental enrichment (EE) and impoverished environment (IE) on cognitive abilities and hippocampal function. However, the specific gene programs driving these changes remain largely unknown.
Read the full summary ↓
In this study, we employed EE and IE paradigms to modulate cognitive abilities in opposing directions in mice. By combining hippocampal microdissection and genetic tagging of neuronal nuclei with genome-wide analyses of gene expression, chromatin accessibility, histone acetylation, and DNA methylation, we uncovered cell type-specific genomic changes induced by EE and IE in CA1 pyramidal neurons and dentate gyrus (DG) granule neurons. This multiomic screen identified the activity-regulated transcription factor AP-1 as a crucial mediator of neuroadaptation to environmental conditions during early life in both neuronal populations, albeit through distinct downstream mechanisms. Conditional deletion of Fos, a core AP-1 subunit, in excitatory neurons hampered EE-induced cognitive enhancement, further underscoring the pivotal role of this transcription factor in neuroadaptation.
Published in
Neuronal type-specific modulation of cognition and AP-1 signaling by early-life rearing conditions
Alaiz-Noya M, Miozzo F, Fuentes-Ramos M et al. · Nature communications 2025 · PMID 41193527 · doi:10.1038/s41467-025-65343-5
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE288334_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1216956 and SRA study SRP560588. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 18 more — browse all 18 samples with per-sample file links →

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