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Differential expression of RhoA gene in SMART-Cas9 knockout macrophages

GSE289266 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/02/11 Platform GPL24247
Summary
We have identified that Ras homolog gene family member A (RhoA) is a pivotal target in inducing osteoclastogenesis in macrophages. Subsequently, we developed a strategy termed Specific Macrophage RhoA Targeting (SMART). In this strategy, phosphatidylserine-enriched macrophage membranes are engineered to deliver CRISPR-Cas9 plasmids containing a macrophage-specific promoter (SMART-Cas9), thereby enabling targeted editing of the RhoA gene in RAW264.7 macrophages. High-throughput transcriptome sequencing was then performed. Transcriptome analysis revealed that ablation of the macrophage RhoA gene significantly inhibited key signaling pathways associated with bone destruction.
Published in
Specific macrophage RhoA targeting CRISPR-Cas9 for mitigating osteoclastogenesis-induced joint damage in inflammatory arthritis
Chen J, Tan J, Wang N et al. · Cell reports. Medicine 2025 · PMID 40239628 · doi:10.1016/j.xcrm.2025.102046
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Also filed as BioProject PRJNA1222299 and SRA study SRP563163. Searching any of these in the dataset finder brings you back here.

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