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Defects in nephrogenesis result in an expansion of the Foxd1+ stromal progenitor population

GSE289696 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/02/22 Platform GPL30172
Summary
Reciprocal signaling interactions coordinate multiple aspects of kidney development. While signals from the stroma have been shown to regulate nephron progenitor cell (NPC) differentiation, much less is known about regulation of the stromal progenitor population. We sought to further examine how defects in the nephron lineage may non-autonomously affect stromal development. To aid in this analysis, we generated single nuclei RNA-seq using 10x genomics from paired E15.5 control kidneys (cre-negative and Six2creTGC) and mutant kidneys (Six2cre;Wt1c/c) to identify additional markers and gene expression changes of the Foxd1+ stromal progenitor population at this timepoint, and validated the expression of early B cell factor 3 (Ebf3), aldehyde oxidase 3 (Aox3), fibroblast activation protein (Fap), insulin growth factor 1 (Igf1), and glutamate ionotropic receptor AMPA type subunit 1 (Gria1) in control and mutant kidneys.
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Direct links to NCBI, no account and no request form: the whole study as GSE289696_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1224207 and SRA study SRP564127. Searching any of these in the dataset finder brings you back here.

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