GEO series

The Absence of Claudin-10 In the Enamel Organ Alters Its Integrity

GSE289840 Mus musculus (mouse) RNA-seq (gene expression) 10 samples Submitted 2026/02/01 Platform GPL19057

At a glance

RNA-seq (gene expression) · mouse · 10 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 10 samples: 10 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.

Summary (written by the submitters)

Enamel, the hardest structure in the body, is affected throughout life by chronic or rare diseases. The study of the underlying pathological mechanisms has revealed new players in enamel formation. Among them, the disorders related to tight junction (TJ) proteins and kidney have been associated with impaired ameloblast function. Pathogenic variants of CLDN10, encoding claudin-10b, a cation transport channel, cause the autosomal recessive HELIX syndrome (Hypohidrosis, Electrolyte imbalance, hypoLacrymia, Ichthyosis, Xerostomia) in which patients experience disabling enamel wear.

Read the full summary ↓

This study explored whether this premature enamel wear is due to intrinsic enamel fragility or xerostomia. In this purpose, we analyzed amelogenesis and enamel in several constitutive or conditional Cldn10 knockout (KO) murine models and human teeth. Our data show that claudin-10 was present in the cell membranes of the stratum intermedium, but not TJs, and that its absence disrupted signaling pathways related to ion transport and pH control. Although the ameloblasts still formed enamel, higher levels of aluminum were found in transplanted Cldn10 KO molars and in human HELIX enamel. These findings suggest that while xerostomia is the main cause of enamel wear, claudin-10 plays a key role in amelogenesis by regulating pH, and its deficiency leads to a loss of enamel organ integrity.

This dataset

Open GSE289840 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE289840_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1225174 and SRA study SRP564629. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Study design

10 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 10 sample titles: 10 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8800021 Mice WT claudin 10 rep 1 ameloblast
GSM8800022 Mice WT claudin 10 rep 2 ameloblast
GSM8800023 Mice KO claudin 10 rep 1 ameloblast
GSM8800024 Mice KO claudin 10 rep 2 ameloblast
GSM8800025 Mice KO claudin 10 rep 3 ameloblast
GSM8800026 Mice WT claudin 10 rep 1 ST
GSM8800027 Mice WT claudin 10 rep 2 ST
GSM8800028 Mice KO claudin 10 rep 1 ST
GSM8800029 Mice KO claudin 10 rep 2 ST
GSM8800030 Mice KO claudin 10 rep 3 ST

Datasets on a similar topic

GSE294054 Obesity accelerates age-related memory deficits and alters white matter tract integrity in Ldlr-/-.Leiden mice 39 samples shares integrity, alters
GSE275499 TEX cell fate and differentiation are shaped by 3D genome reorganization and chromatin architecture integrity [RNA-seq] 18 samples shares integrity, organ
GSE226610 Transcriptomic analysis of enamel organ in a mouse model of Loeys-Dietz Syndrome Type II harboring a G357W mutation in the Tgfbr2 gene 13 samples shares enamel, organ
GSE284165 Reorganization of PBAF/cBAF remodelers on H3.3 chromatin regulates BMAL1 activity in the absence of circadian negative feedback 47 samples shares absence, organ
GSE217767 ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss 20 samples shares integrity, alters
GSE284090 TEX cell fate and differentiation are shaped by 3D genome reorganization and chromatin architecture integrity [D3] 18 samples shares integrity, organ
GSE217765 ARF alters PAF1 complex integrity to selectively repress oncogenic transcription programs upon p53 loss 14 samples shares integrity, alters
GSE326200 Absence of Clec1a in murine cDC1s decrease IFN responses to necrotic cells 237 samples shares absence

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.