RNA-seq Profiling of Response to Cisplatin and Gemcitabine Chemotherapy in Advanced Urothelial Carcinoma: An External Validation Cohort

This dataset includes RNA sequencing data from formalin-fixed paraffin-embedded (FFPE) tumor samples of advanced urothelial carcinoma patients treated with cisplatin and gemcitabine combination chemotherapy at Pusan National University Yangsan Hospital (PNUYH). RNA was extracted using the RNeasy FFPE kit (Qiagen) and sequenced on the Illumina platform using the SureSelectXT RNA Direct Reagent Kit (Agilent) for paired-end sequencing. Raw sequencing data underwent quality control and trimming using Trimmomatic-0.39-1, followed by sequence alignment using STAR 2.7.8a and expression quantification using RSEM 1.3.3. Treatment responses were evaluated according to RECIST v1.1 criteria. This dataset serves as an external validation cohort for a deep learning-based chemotherapy response prediction model in urothelial carcinoma.