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RNA-seq analysis of BMSCs differentiated into osteoblasts treated with Hepa 1-6-derived CM and control medium

GSE290364 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/10/01 Platform GPL24247
Summary
In certain pathological conditions, there exists a liver-bone axis. However, the intricacies of liver-bone communication in HCC growth and metastasis has so far remained incompletely understood. The conditioned medium from Hepa 1-6 cells (Hepa 1-6-derived CM), compared with the control medium, could enhance osteoblast differentiation of Bone Marrow Mesenchymal Stromal Cells (BMSCs). To investigate the mechanism by which remote HCC cells mediate the osteogenic activity of tumor-bearing mice, we performed bulk RNA-seq on BMSCs differentiated into osteoblasts treated with Hepa 1-6-derived CM and control medium. Following the analysis of upregulated genes analysis, we identified a significant elevation in C-X-C chemokine motif ligand 5 (Cxcl5), which encodes CXCL5 and could mediate osteogenic activity.
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Direct links to NCBI, no account and no request form: the whole study as GSE290364_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 6 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1227558 and SRA study SRP566016. Searching any of these in the dataset finder brings you back here.

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