Comprehensive profiling of N6-methyladnosine (m6A) readouts reveals novel m6A readers that regulate human embryonic stem cell differentiation [GLORI-seq]
RNA-seq (gene expression) · human · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Samples
- 12 samples: 6 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Comprehensive Profiling of N(6)-methyladnosine (m(6)A) Readouts Reveals Novel m(6)A Readers That Regulate Human Embryonic Stem Cell DifferentiationAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE290408_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1227924 and SRA study SRP566193. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 6 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8812247 A549, WT, rep1 A549, WT,
- GSM8812248 A549, WT, rep2 A549, WT,
- GSM8812249 hESC, WT, rep1 hESC, WT,
- GSM8812250 hESC, WT, rep2 hESC, WT,
- GSM8812251 JURKAT, WT, rep1 JURKAT, WT,
- GSM8812252 JURKAT, WT, rep2 JURKAT, WT,
- GSM9307207 A549, METTL3 KD, rep1 A549, METTL3 KD,
- GSM9307208 A549, METTL3 KD, rep2 A549, METTL3 KD,
- GSM9307209 hESC, METTL3 KD, rep1 hESC, METTL3 KD,
- GSM9307210 hESC, METTL3 KD, rep2 hESC, METTL3 KD,
- GSM9307211 JURKAT, METTL3 KD, rep1 JURKAT, METTL3 KD,
- GSM9307212 JURKAT, METTL3 KD, rep2 JURKAT, METTL3 KD,
- GSE288140 HDAC2-Mediated Recruitment of METTL3 to Chromatin Regulates Human Embryonic Stem Cell Differentiation 74 samples shares embryonic, regulate, stem
- GSE282179 SPT6 Regulates H3K14Ac Deposition in Mouse Embryonic Stem Cells [ChIP-seq-HEK293T] 24 samples shares embryonic, regulate, stem
- GSE256456 O-GlcNAcase Regulates Pluripotency States of Human Embryonic Stem Cells 12 samples shares embryonic, regulate, stem
- GSE275739 CHD7 Regulates Definitive Endodermal and Mesodermal Development from Human Embryonic Stem Cells [ATACseq] 10 samples shares embryonic, regulate, stem
- GSE201709 Multiomic profiling of vascular endothelial cell differentiation from human embryonic stem cells 130 samples shares embryonic, stem
- GSE242906 Transcription factor ZNF263 primes human embryonic stem cells for pluripotency dissolution and early differentiation 81 samples shares embryonic, stem
- GSE328191 Multiomic profiling of vascular endothelial cell differentiation from human embryonic stem cells [ChIP-seq] 80 samples shares embryonic, stem
- GSE249035 Sex-biased Gene Expression During Neural Differentiation of Human Embryonic Stem Cells 72 samples shares embryonic, stem
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.