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SMART-seq of MG lineages in MNU-injured retinas

GSE290470 Mus musculus Expression profiling by high throughput sequencing 5 samples Submitted 2025/03/14 Platform GPL21626
Summary
We further investigated injury-induced gene expression changes selectively in MG cell lineages. The tdTom-labeled MG and their descendant cells were isolated via FACS from uninjured or MNU-injured Glast-CreERT;R26tdTom/+ mouse retinas, which were infected with either AAV2-Ctrl Ab or AAV2-alpha Prox1, and mRNA expression patterns of individual cells were analyzed using SMART (Switching Mechanism At the 5' end of RNA Template) sequencing methods. We could identify a lineage flow from resting MG to RPC-like cells via activated MG, along with pseudo-time progression.
Published in
Restoration of retinal regenerative potential of Müller glia by disrupting intercellular Prox1 transfer
Lee EJ, Kim M, Park S et al. · Nature communications 2025 · PMID 40133314 · doi:10.1038/s41467-025-58290-8
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Also filed as BioProject PRJNA1226501 and SRA study SRP565571. Searching any of these in the dataset finder brings you back here.

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