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RNA-sequencing of HCMV-infected ARPE-19 and MCF10A cells

GSE290998 Homo sapiens (human) RNA-seq (gene expression) 48 samples Submitted 2025/03/11 Platform GPL24676
At a glance

RNA-seq (gene expression) · human · 48 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
48 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Summary (written by the submitters)
In this study, viral and cellular gene expression profiles were acquired after infecting ARPE-19 and MCF10A cells with strain TB40/E-BAC4 of human cytomegalovirus. ARPE-19 cells are permissive for HCMV infection, while MCF10A cells are semi-permissive. This data was acquired to study the effects of epithelial and mesenchymal cell states on HCMV infection.
Read the full summary ↓
Despite being derived from a primary retinal epithelial explant, ARPE-19 cells require long periods of high confluency to display epithelial characteristics. Under the conditions used in this experiment, ARPE-19 cells are phenotyically mesenchymal, as shown in Golconda et al, Viruses, 2024. MCF10A cells display strong epithelial characteristics under the conditions used here.
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE290998_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1231357 and SRA study SRP567771. Searching any of these in the dataset finder brings you back here.

Samples in this study

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+ 48 more — browse all 48 samples with per-sample file links →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.