← BioTransfer GEO Dataset Finder
GEO series

2’-O-Methyl-guanosine 3-base RNA fragments mediate essential natural TLR7/8 antagonism

GSE291606 Mus musculus Expression profiling by high throughput sequencing 9 samples Submitted 2025/12/09 Platform GPL30172
Summary
Daily phagocytic clearance of billions of apoptotic cells is fundamental to the maintenance of homeostasis and is normally immune silent. Toll-like receptors (TLR) 7 and 8 are phagosomal innate immune sensors known to instigate inflammation in response to guanosine, uridine, and ~2-3 base long degradation products from endosomal RNAs. Exactly how TLR7 and TLR8 evade activation by degradation products from host-RNAs is currently unknown. The activity of the mouse TLR7 inhibitory sequence GGC-v1 was tested on primary bone marrow-derived macrophages (BMDMs) derived from Tlr7Y264H gain-of-function mutant mice. This Tlr7Y264H gain of function mutation results in constitutive engagement of TLR7 through an increased affinity for guanosine. Overnight treatment of Tlr7Y264H mutant BMDMs with GGC-v1 or the small molecule TLR7/8 inhibitor Enpatoran led to significant down regulation of 20 out of the 22 genes regulated by GGC-v1 that were also down-regulated by Enpatoran, indicating that TLR7 sensing was effectively dampened.
Published in
2'-O-Methyl-guanosine RNA fragments antagonize TLR7 and TLR8 to limit autoimmunity
Alharbi AS, Sapkota S, Zhang Z et al. · Nature immunology 2026 · PMID 41667621 · doi:10.1038/s41590-026-02429-2
This dataset
Download

Direct links to NCBI, no account and no request form: the whole study as GSE291606_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1234485 and SRA study SRP569506. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 9 more — browse all 9 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.