← BioTransfer GEO Dataset Finder
GEO series

Proton-activated chloride channel 1 is essential for innate host defense against bacterial sepsis

GSE292930 Mus musculus (mouse) RNA-seq (gene expression) 24 samples Submitted 2026/03/17 Platform GPL34290
At a glance

RNA-seq (gene expression) · mouse · 24 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
24 samples: 6 × Bone marrow-derived macrophage, wild type, unstimulated control, vs 6 × Bone marrow-derived macrophage, Pacc1-/-, unstimulated control, vs 6 × Bone marrow-derived macrophage, wild type, stimulated, vs 6 × Bone marrow-derived macrophage, Pacc1-/-, stimulated,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Proton-activated chloride channel 1 is essential for innate host defense against bacterial sepsisProceedings of the National Academy of Sciences of the United States of America · 2026
Summary (written by the submitters)
Bacterial sepsis remains a devastating clinical problem. Here, we describe a protective role for the recently discovered acid-sensitive, proton-activated chloride channel, PACC1 (PAC/ASOR/TMEM206), during sepsis. Initially, we found PACC1 was enriched in healthy human and mouse mononuclear phagocytes, particularly macrophages, and differentially regulated by inflammatory stimuli, suggesting PACC1 involvement in innate immunity.
Read the full summary ↓
To further investigate, we generated de novo Pacc1 knockout (−/−) mice, which presented without major immunologic abnormalities at baseline. Compared to wild type (WT), Pacc1−/− myeloid cells showed normal phagocytic uptake of acid-insensitive Escherichia coli BioParticles, but impaired development of the acidifying phagolysosome using acid-sensitive E. coli BioParticles. Transcriptomic profiling of Pacc1−/− macrophages revealed dysregulated phagolysosomal and cytokine networks (e.g., interferons). Because phagolysosomal bacterial clearance is essential to resolve infection, we challenged Pacc1−/− mice with intraperitoneal gram-negative E. coli sepsis. Pacc1−/− mice displayed increased bacterial burden, immune cell infiltration, inflammation, and lethality. In contrast, phagocytosis-independent E. coli lipopolysaccharide (LPS)-induced endotoxemia yielded comparable WT and Pacc1−/− survival, as well as similar inflammatory responses. Finally, we engineered Pacc1-floxed (fl/fl) mice crossed with a myeloid lineage Cre-deleter strain to interrogate myeloid cell–intrinsic PACC1 in vivo. Consistent with a predominate role for PACC1 during phagocytosis and bacterial clearance in these cells, LysM-Cre/Pacc1fl/fl mice exhibited impaired E. coli sepsis survival but indifferent endotoxemia phenotypes. In conclusion, PACC1 links sterilizing phagolysosomal activity with immune networks in sepsis pathobiology.
Published in
Proton-activated chloride channel 1 is essential for innate host defense against bacterial sepsis
Garo LP, Brueck K, Walachowski S et al. · Proceedings of the National Academy of Sciences of the United States of America 2026 · PMID 41941636 · doi:10.1073/pnas.2515768123
This dataset
Open GSE292930 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE292930_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1242180 and SRA study SRP573442. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41941636) ↗
Study design
6 × Bone marrow-derived macrophage, wild type, unstimulated control, vs 6 × Bone marrow-derived macrophage, Pacc1-/-, unstimulated control, vs 6 × Bone marrow-derived macrophage, wild type, stimulated, vs 6 × Bone marrow-derived macrophage, Pacc1-/-, stimulated,

Supports a between-group comparison across 24 samples.

How we read this: 4 replicated groups read from 24 sample titles; they account for 24 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.