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Uncovering common transcriptional features shared by mature PMN-MDSCs and tumor-associated neutrophils in humans

GSE293018 Homo sapiens Expression profiling by high throughput sequencing; Other 7 samples Submitted 2025/07/09 Platform GPL18573
Summary
In this study, we performed single-cell transcriptomic profiling of mature PMN-MDSCs (mPMN-MDSCs) from non-small cell lung cancer (NSCLC) patients and identified a distinct cell cluster (NSCLC c6) exhibiting immunosuppressive and protumor features. Comparative analysis with publicly available single-cell RNA sequencing (scRNA-seq) datasets of tumor-associated neutrophils (TANs) revealed shared transcriptomic features between the identified NSCLC mPMN-MDSC and TAN clusters. These transcriptomic features included the expression of common genes, activation of the hypoxia signaling pathway, and metabolic reprogramming. Additionally, scRNA-seq analysis of mature immunosuppressive neutrophils from G-CSF-treated donors (GDs) – which are a reliable model of circulating mPMN-MDSCs - demonstrated similar dysregulation of hypoxia and metabolic pathways, further reinforcing the existence of transcriptomic similarities between circulating mPMN-MDSCs and TANs.
Published in
Uncovering common transcriptional features shared by mature peripheral blood PMN-MDSCs and tumor-infiltrating neutrophils in humans
Lattanzi C, Bianchetto-Aguilera F, Donini M et al. · Oncoimmunology 2025 · PMID 40590753 · doi:10.1080/2162402X.2025.2521396
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Direct links to NCBI, no account and no request form: the whole study as GSE293018_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 7 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1242361 and SRA study SRP573357. Searching any of these in the dataset finder brings you back here.

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