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Regulation of germinal center B-cell homeostasis by Deltex-1 (Dtx1) [scRNA]

GSE293902 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2026/04/04 Platform GPL24247
Summary
Germinal centers (GC) are sites for the clonal expansion and affinity maturation of B-cells that is coordinated by interaction with follicular dendritic cells (fDCs) and T follicular helper (TFH) cells. Loss of GC homeostasis can contribute to the development of B-cell malignancies and autoimmunity. Here we sought to investigate the GC-specific function of Deltex-1 (Dtx1), a gene that is highly expressed in GC B-cells and is a prominent target of aberrant somatic hypermutation. Dtx1 conditional knock-out (cKO) led to a competitive advantage in the GC reaction and marked GC hyperplasia accompanied by expansion of, and dependence upon, the fDC network. Dtx1 cKO GC B-cells bore transcriptional evidence of Notch and Myc pathway activation, and genetic inhibition of Notch activity led to resolution of GC hyperplasia. Together, these data implicate Dtx1 as a critical regulator of fDC-derived Notch signaling and GC homeostasis.
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Direct links to NCBI, no account and no request form: the whole study as GSE293902_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1247400 and SRA study SRP576831. Searching any of these in the dataset finder brings you back here.

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