Single cell transcriptomic analysis of splenic gamma delta T cells, thymic gamma delta T cells and thymic DN1-DN2-DN3 cells from WT and GFI1 KO mice.

GFI1 is a transcription factor known to be essential for hematopoiesis and lymphoid cell generation. We have recently found that gamma delta T cells express GFI1 and its absence can impact gamma delta T cell formation. In order to identify how the lack of GFI1 can affect the development of thegamma delta T cell subsets, we have performed a single cell sequencing approach using 10X genomics scRNAseq on splenic gamma delta T cells, thymic gamma delta T cells and a pool of thymic DN1-2-3 (DN) cells from WT and GFI1 KO mice. The sc RNA-seq highlighted differential transcriptomic changes between WT and GFI1 KO cells showing enrichment or absence of specific subsets of gamma delta cells and their thymic progenitors.