RNA profiling of wild-type Osteosarcoma (U2OS) cells and its KO derivative (PERK KO) upon Palbociclib treatment for 4 days
RNA-seq (gene expression) · human · 9 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Homo sapiens (human)
- Topic
- OsteosarcomaThis series is part of our curated briefing on it.
- Samples
- 9 samples
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE294635_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1250979 and SRA study SRP578691. Searching any of these in the dataset finder brings you back here.
The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.
+ 9 more — browse all 9 samples with per-sample file links →
- GSE250492 Effect of over expression of MED13 IDR in human osteosarcoma U2OS cell line 6 samples same disease briefing · shares u2os, osteosarcoma
- GSE268088 The effect of ZFP36L1 deficiency on gene expression in the U2OS osteosarcoma cancer cell line 6 samples same disease briefing · shares u2os, osteosarcoma
- GSE275890 Establishment of U2OS osteosarcoma cell line with GREM1 knockdown 4 samples same disease briefing · shares u2os, osteosarcoma
- GSE282643 Establishment of U2OS osteosarcoma cell line with GREM1 knockdown II 4 samples same disease briefing · shares u2os, osteosarcoma
- GSE278302 Enhanced combination treatment on osteosarcoma by mitigating drug-induced oncogenic signaling pathway activation 12 samples same disease briefing · shares osteosarcoma, treatment
- GSE259286 In vitro modeling of nutritional and mitochondrial-targeted therapies for osteosarcoma 98 samples same disease briefing · shares osteosarcoma
- GSE253548 The whole transcriptome analysis using FFPE and fresh tissue samples identifies the molecular fingerprint of osteosarcoma 90 samples same disease briefing · shares osteosarcoma
- GSE152048 Single cell analysis of osteosarcoma tissues 76 samples same disease briefing · shares osteosarcoma
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.