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Single Cell RNA-seq of PgrCre/+Srfflox/flox and Srfflox/flox mouse uterus at gestation day 3.5

GSE294752 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/09/30 Platform GPL24247
Summary
Single cell RNA-seq analysis demonstrated dramatic changes in adult PgrCre/+Srfflox/flox endometrial cell populations compared to Srf flox/flox controls. Uterine Srf ablation caused myeloid immune cell infiltration, and differential expression analysis demonstrated that PgrCre/+Srfflox/flox stromal fibroblasts downregulated progesterone-regulated genes and growth response genes. Proliferating fibroblast populations shifted from G2-M phase-dominant in controls to S-G2 phase-dominant in PgrCre/+Srfflox/flox uteri. PgrCre/+Srfflox/flox epithelial cells overexpressed estrogenic and innate inflammatory genes.
Published in
Serum response factor is essential for endometrial function and prevention of inflammatory fibrosis
Marquardt RM, Grimm SA, Wu SP et al. · Proceedings of the National Academy of Sciences of the United States of America 2025 · PMID 41150713 · doi:10.1073/pnas.2510060122
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Also filed as BioProject PRJNA1251551 and SRA study SRP579016. Searching any of these in the dataset finder brings you back here.

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