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Colony morphology altered by Myh9 deficiency uncoupled from pluripotency

GSE294784 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2025/12/02 Platform GPL21273
Summary
The naïve mouse embryonic stem cells, characterized by high nuclear/cytoplasmic ratio, and dome-shaped colonies with smoothly contoured edges, exhibit self-renewal and pluripotency. However, it is unclear about the biological significance of dome-like morphology in relation to pluripotency. Here, we generate stable ES cell lines with a flat morphology, capable of sustained monolayer growth and efficient single-cell clonogenic capacity, by knocking out non-muscle myosin heavy chain IIA. Notably, even when deprived of the typical dome-like colony morphology, the ES cells retain their self-renewal capacity and pluripotency to differentiate into the three germ layers. Our data disprove the long-standing paradigm in stem cell field that the dome-shaped colony architecture is intrinsically linked to naïve pluripotency, instead demonstrating that core pluripotency circuitry operates independently of this morphological constraint.
Published in
Breaking the link between morphology and potency for mESCs
Fan Y, Wang X, Zhai Z et al. · Cell & bioscience 2025 · PMID 41131638 · doi:10.1186/s13578-025-01497-5
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Also filed as BioProject PRJNA1251601 and SRA study SRP579044. Searching any of these in the dataset finder brings you back here.

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