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Single-cell RNA sequencing on TILs from 4T1 mouse model treated with STING agonist deliveredy by PEG biopsy marker shows activation of antigen presenting cells and macrophages but does not show T cell clonality.

GSE294854 Mus musculus Expression profiling by high throughput sequencing; Other 4 samples Submitted 2025/04/21 Platform GPL34328
Summary
To better characterize the TME effects of ADU-S100 delivered by the PEG, we performed single cell RNA sequencing (scRNA-seq) on CD45+ immune cells from 4T1 tumors 14 days after treatment. We found that antigen presenting cell (APC) and macrophage clusters exhibited robust upregulation of IFN-induced genes by ADU-S100, as well as significant enrichment for hallmark IFN gene signatures. To explore whether immune escape could be secondary to a failure of STING agonist to induce T-cell clonality and expansion, we further performed T-cell receptor sequencing (TCR-seq) on these CD45+ immune cells from animals treated with PEG markers with PBS or 100 µg ADU-S100. We then analyzed the frequency of unique clonotypes that were expanded following PBS versus ADU-S100 treatment. In both groups, there was no evidence for clonotype expansion.
Published in
Immune targeting of triple-negative breast cancer through a clinically actionable STING agonist-CAR T cell platform
Zhang Y, Li Z, Ritter J et al. · Cell reports. Medicine 2025 · PMID 40543510 · doi:10.1016/j.xcrm.2025.102198
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Also filed as BioProject PRJNA1251700 and SRA study SRP579144. Searching any of these in the dataset finder brings you back here.

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