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Loss of EGFR prevents the synergistic early-phase impact of obesity-associated metabolic and humoral stress on primary VSMC – stressor synergy, sex-dependence

GSE294868 Mus musculus (mouse) RNA-seq (gene expression) 69 samples Submitted 2025/12/10 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 69 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
69 samples: 12 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Early-phase impact of obesity-associated stress on murine vascular smooth muscle cells depends on EGFR and sexCommunications biology · 2025
Summary (written by the submitters)
RNAsequencing was performed for murine primary aortic smooth muscle cells (SMC) and endothelial cells (EC) from wildtype animals (WT) and animals with conditional EGFR knockout in VSMC (KOsmc) after exposing the cells to obestity-associated stress factors.
Published in
Early-phase impact of obesity-associated stress on murine vascular smooth muscle cells depends on EGFR and sex
Dubourg V, Rabe S, Nasiri-Ansari N et al. · Communications biology 2025 · PMID 41429904 · doi:10.1038/s42003-025-09416-7
This dataset
Open GSE294868 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE294868_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1251881 and SRA study SRP579351. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41429904) ↗
Study design
12 conditions, mostly with about 4 replicates each
EC_WT_HG+FFS_G ×10 EC_WT_ALL_G ×10 EC_WT_Control_G ×9 EC_WT_AII+NA_G ×9 VSMC_WT_Control_G ×4 VSMC_WT_HG+FFS_G ×4 VSMC_WT_AII+NA_G ×4 VSMC_WT_ALL_G ×4 +4 more

Supports a between-group comparison across 69 samples.

How we read this: 12 replicated groups read from 69 sample titles; they account for 69 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 29 more — browse all 69 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.