GEO series

Dietary restriction mitigates vascular aging, modulates the cGAS-STING pathway and reverses macrophage-like VSMC phenotypes in progeroid DNA-repair-deficient Ercc1Δ/- mice

GSE294678 Mus musculus (mouse) RNA-seq (gene expression) 29 samples Submitted 2025/04/16 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 29 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 29 samples: 29 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Dietary Restriction Mitigates Vascular Aging, Modulates the cGAS-STING Pathway and Reverses Macrophage-Like VSMC Phenotypes in Progeroid DNA-Repair-Deficient Ercc1(Δ) (/-) MiceAging cell · 2025

Summary (written by the submitters)

Aging is a major risk factor for cardiovascular diseases and the accumulation of DNA damage significantly contributes to the aging process. This study aimed to identify the underlying molecular mechanisms of vascular aging in DNA-repair-deficient progeroid Ercc1Δ/- mice and to explore the therapeutic effect of dietary restriction (DR).

Read the full summary ↓

RNA sequencing analysis revealed that DR reversed gene expression of vascular aging processes including extracellular matrix remodeling in the Ercc1Δ/- aorta. Notably, this analysis indicated the presence of macrophage-like vascular smooth muscle cells (VSMCs) and suggested cGAS-STING pathway activation. The presence of macrophage-like VSMCs and increased STING1 expression were confirmed in Ercc1Δ/- aortic tissue and were both reduced by DR. In vitro, cisplatin-induced DNA damage activated the cGAS-STING pathway in Ercc1Δ/- VSMCs but not in wildtype VSMCs. These findings identify involvement of the cGAS-STING pathway in DNA damage driven vascular aging and underscore the therapeutic benefits of DR for vascular aging. Furthermore, upstream regulator analysis revealed compounds that may replicate the beneficial effects of DR, providing promising leads for further investigation.

Published in

Dietary Restriction Mitigates Vascular Aging, Modulates the cGAS-STING Pathway and Reverses Macrophage-Like VSMC Phenotypes in Progeroid DNA-Repair-Deficient Ercc1(Δ) (/-) Mice

Stefens SJM, van der Linden J, Heredia-Genestar JM et al. · Aging cell 2025 · PMID 40279334 · doi:10.1111/acel.70062

This dataset

Open GSE294678 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE294678_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1251075 and SRA study SRP578834. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40279334) ↗

Study design

29 samples, each with a distinct title — no replicated groups

Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.

How we read this: Read from 29 sample titles: 29 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8915834 Sample 024, Ercc1 +/+, aortic arch, female, 6 weeks, AL
GSM8915835 Sample 001, Ercc1 +/+, aortic arch, male, 6 weeks, AL
GSM8915836 Sample 019, Ercc1 +/+, aortic arch, male, 6 weeks, AL
GSM8915837 Sample 011, Ercc1 +/+, aortic arch, female, 6 weeks, AL
GSM8915838 Sample 009, Ercc1 +/+, aortic arch, male, 6 weeks, AL
GSM8915839 Sample 002, Ercc1 d/-, aortic arch, male, 6 weeks, AL
GSM8915840 Sample 008, Ercc1 d/-, aortic arch, female, 6 weeks, AL
GSM8915841 Sample 015, Ercc1 d/-, aortic arch, male, 6 weeks, AL
GSM8915842 Sample 023, Ercc1 d/-, aortic arch, female, 6 weeks, AL
GSM8915843 Sample 027, Ercc1 d/-, aortic arch, male, 6 weeks, AL
GSM8915844 Sample 003, Ercc1 +/+, aortic arch, female, 22 weeks, AL
GSM8915845 Sample 012, Ercc1 +/+, aortic arch, male, 22 weeks, AL
GSM8915846 Sample 018, Ercc1 +/+, aortic arch, female, 22 weeks, AL
GSM8915847 Sample 025, Ercc1 +/+, aortic arch, male, 22 weeks, AL
GSM8915848 Sample 020, Ercc1 +/+, aortic arch, male, 22 weeks, AL
GSM8915849 Sample 007, Ercc1 +/+, aortic arch, male, 22 weeks, DR
GSM8915850 Sample 017, Ercc1 +/+, aortic arch, female, 22 weeks, DR
GSM8915851 Sample 010, Ercc1 +/+, aortic arch, male, 22 weeks, DR
GSM8915852 Sample 028, Ercc1 +/+, aortic arch, male, 22 weeks, DR
GSM8915853 Sample 021, Ercc1 +/+, aortic arch, female, 22 weeks, DR
GSM8915854 Sample 005, Ercc1 d/-, aortic arch, female, 22 weeks, AL
GSM8915855 Sample 013, Ercc1 d/-, aortic arch, male, 22 weeks, AL
GSM8915856 Sample 004, Ercc1 d/-, aortic arch, female, 22 weeks, AL
GSM8915857 Sample 026, Ercc1 d/-, aortic arch, male, 22 weeks, AL
GSM8915858 Sample 006, Ercc1 d/-, aortic arch, male, 22 weeks, DR
GSM8915859 Sample 014, Ercc1 d/-, aortic arch, female, 22 weeks, DR
GSM8915860 Sample 029, Ercc1 d/-, aortic arch, male, 22 weeks, DR
GSM8915861 Sample 016, Ercc1 d/-, aortic arch, male, 22 weeks, DR
GSM8915862 Sample 022, Ercc1 d/-, aortic arch, female, 22 weeks, DR

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