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RNA sequencing of Tcf7 and Prdm1 deficient T cells [RNAseq_Tcf7Prdm1DKO]

GSE294881 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2025/10/02 Platform GPL34290
Summary
The transcription factors Tcf7 (encoding TCF1) and Prdm1 (encoding Blimp1) exhibit opposing effects on T cell differentiation. While TCF1 promotes the maintenance of T progenitor exhausted cells, Blimp1 drives their differentiation into terminally exhausted effector cells. However, the impact of the combined deletion of these factors on CD8 T cell differentiation remains to be defined. We found that CD8 T cells in which TCF1 and Blimp1 are both ablated exhibit a TPEX surface phenotype in chronic infection but fail to expand in recall settings. To better understand how these factors poise T cells to respond to antigen encounter, bulk RNA-sequencing was performed on TPEX cells from WT and Tcf7 Prdm1 DKO CD8 T cells.
Published in
The transcriptional repressor BLIMP1 enforces TCF-1-dependent and -independent restriction of the memory fate of CD8(+) T cells
Murphy MK, McCullen M, Deffenbaugh JL et al. · Immunity 2025 · PMID 41043414 · doi:10.1016/j.immuni.2025.09.008
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Also filed as BioProject PRJNA1251139 and SRA study SRP578867. Searching any of these in the dataset finder brings you back here.

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