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DNA-damage dependent association of Orc6 to ISWI during S-phase modulates chromatin remodeling [CUT_and_RUN]

GSE295487 Homo sapiens (human) ChIP-seq / ATAC-seq (genome binding and open chromatin) 34 samples Submitted 2026/05/19 Platform GPL24676
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · human · 34 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Homo sapiens (human)
Samples
34 samples: 14 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
DNA-damage dependent interaction of Orc6 to SMARCA1 in S-phase modulates chromatin remodelingNucleic acids research · 2026
Summary (written by the submitters)
The human Origin Recognition Complex subunit 6 has recently garnered significant attention because of its dispensability in the licensing of origins. The loss of human Orc6 doesn’t impact MCM loading in vivo, while in vitro reconstitution experiments have demonstrated that MCM loading can occur without Orc6, but could be stimulated upon the addition of Orc6.
Read the full summary ↓
We recently reported an unexpected role for hOrc6, in promoting S-phase progression post pre-RC assembly and DNA damage response (DDR). Using CUT&RUN to map the binding of Orc6 genome-wide, we find that Orc6 is present at replication origins and at sites of DNA damage during S-phase. Upon encountering oxidative damage in S-phase, Orc6 associates with chromatin remodelers, SMARCA1/SNF2L, also a component of the replication fork. Loss of Orc6 shows enhanced genome-wide association of SMARCA1 to Orc6-bound sites, concomitant with chromatin remodeling at these sites supporting the model that Orc6 is a negative regulator of SMARCA1 and constrains its localization. Upon DNA damage, enhanced association of Orc6 to SMARCA1 prevents chromatin remodeling activity in order to halt replication progression. We propose that during S-phase, Orc6 is a barrier factor, which limits the activity of chromatin remodelers at replication and repair sites.
Published in
DNA-damage dependent interaction of Orc6 to SMARCA1 in S-phase modulates chromatin remodeling
Liu D, Mishra M, Wang Y et al. · Nucleic acids research 2026 · PMID 42363755 · doi:10.1093/nar/gkag582
This dataset
Open GSE295487 in finder → Find more human ChIP / ATAC / CUT&Tag datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE295487_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1254958 and SRA study SRP580954. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 42363755) ↗
Study design
14 conditions, mostly in duplicate
U2OS,siGl3,Non-treated,SMARCA1, ×2 U2OS,siGl3,Non-treated,OGG1, ×2 U2OS,siGl3,Non-treated,Orc6, ×2 U2OS,siGl3,5mM H2O2 30min,SMARCA1, ×2 U2OS,siGl3,5mM H2O2 30min,OGG1, ×2 U2OS,siGl3,5mM H2O2 30min,Orc6, ×2 U2OS,siOrc6,Non-treated,SMARCA1, ×2 U2OS,siOrc6,Non-treated,OGG1, ×2 +12 more

Supports a between-group comparison across 28 samples.

How we read this: 14 replicated groups read from 34 sample titles; they account for 28 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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