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Ischemic stroke of different etiologies undergoing mechanical thrombectomy

GSE296028 Homo sapiens (human) RNA-seq (gene expression) 30 samples Submitted 2025/12/02 Platform GPL30173
At a glance

RNA-seq (gene expression) · human · 30 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Homo sapiens (human)
Samples
30 samples: 10 × RNA-Seq Atero vs 10 × RNA-Seq Cardio vs 10 × RNA-Seq ESUSGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Transcriptomic profiling of thrombus-derived extracellular vesicles reveals PECAM-1 as a potential inflammatory marker for cardioembolic stroke patientsJournal of neuroinflammation · 2025
Summary (written by the submitters)
Accurate ischemic stroke (IS) etiologic diagnosis provides an appropriate secondary prevention strategy and better outcome. Currently, 45% of IS are cryptogenic urging to find novel approaches to enhance diagnostic precision and secondary stroke prevention. OBJETIVE: To study the transcriptomic content of thrombus extracellular vesicles (EVs) to delineate the molecular profile and potential biomarkers of IS etiologies.
Read the full summary ↓
RESULTS:30 consecutive patients with moderate or severe ischemic stroke (IS) and different IS etiologies were included to perform the transcriptomic analysis of thrombus EVs comprising: atherothrombotic (AT, n=10), Cardioembolic (CE, n=10), and embolic stroke of undetermined source (ESUS, n=10). The RNASeq analysis of EVs detected clusters of differentially expressed genes (DEGs) related to IS etiologies.
Published in
Transcriptomic profiling of thrombus-derived extracellular vesicles reveals PECAM-1 as a potential inflammatory marker for cardioembolic stroke patients
Machado FJD, Marta-Enguita J, Herrera M et al. · Journal of neuroinflammation 2025 · PMID 41068926 · doi:10.1186/s12974-025-03555-8
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE296028_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1257339 and SRA study SRP582404. Searching any of these in the dataset finder brings you back here.

Study design
10 × RNA-Seq Atero vs 10 × RNA-Seq Cardio vs 10 × RNA-Seq ESUS

Supports a between-group comparison across 30 samples.

How we read this: 3 replicated groups read from 30 sample titles; they account for 30 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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