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Intracellular Delivery of Bone Nanoparticles to Mitigate Irradiation-Induced Damage in Bone Marrow Mesenchymal Stem Cells

GSE296750 Homo sapiens Expression profiling by high throughput sequencing 9 samples Submitted 2025/11/01 Platform GPL34281
Summary
This study evaluated the therapeutic potential of intracellularly delivered bone-derived nanoparticles (BPs) in mitigating Ionizing radiation (IR)-induced BMSCs damage. We found that IR exposure caused significant BMSCs dysfunction, including reduced proliferation, increased apoptosis, persistent DNA damage, and a shift toward adipogenic differentiation. Treatment with BPs led to efficient intracellular uptake, improved cell morphology, enhanced proliferation, reduced apoptosis, and preservation of balanced differentiation capacity. Transcriptomic analysis via RNA sequencing revealed that BPs restored key molecular pathways disrupted by IR, particularly those involved in cell cycle regulation, extracellular matrix (ECM) remodeling, and apoptosis. By reversing these transcriptional impairments, BPs supported genomic stability and the regenerative function of BMSCs.
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Direct links to NCBI, no account and no request form: the whole study as GSE296750_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1261001 and SRA study SRP584289. Searching any of these in the dataset finder brings you back here.

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