GEO series

PIEZO1 mediates mechanical reprogramming of neutrophils for proangiogenic specialization in the lung

GSE297134 Mus musculus (mouse) RNA-seq (gene expression) 23 samples Submitted 2025/05/19 Platform GPL24247

At a glance

RNA-seq (gene expression) · mouse · 23 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 23 samples: 9 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: PIEZO1 mediates mechanical reprogramming of neutrophils for proangiogenic specialization in the lungThe Journal of clinical investigation · 2025

Summary (written by the submitters)

Neutrophils are the most abundant immune cells that constantly patrol or marginate inside vascular beds to support immune homeostasis. The extent to which neutrophils undergo reprogramming in response to the changes in vascular architecture and the resultant biological implications of such adaptations remain unclear. Here, we performed intravital imaging and transcriptional profiling to investigate neutrophil behavior across different tissues.

Read the full summary ↓

Our findings revealed that neutrophils had significant deformability and spontaneous calcium signaling while navigating through the narrow pulmonary vessels. Pulmonary neutrophils exhibited unique transcriptional profiles and were specialized for proangiogenic functions. We found that the mechanosensitive ion channel PIEZO1 was essential for neutrophil reprogramming. Deletion of Piezo1 in neutrophils ablated the lung-specific proangiogenic transcriptional signature and impaired capillary angiogenesis in both physiological and pathological conditions. Collectively, these data show that mechanical adaptation of neutrophils within the pulmonary vasculature drives their reprogramming in the lungs and promotes pulmonary vascular homeostasis.

Published in

PIEZO1 mediates mechanical reprogramming of neutrophils for proangiogenic specialization in the lung

Wang J, Zhao W, Bai W et al. · The Journal of clinical investigation 2025 · PMID 40454475 · doi:10.1172/JCI183796

This dataset

Open GSE297134 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE297134_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1262857 and SRA study SRP585355. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 40454475) ↗

Study design

9 conditions, mostly in triplicate

wt mouse lung ×3 wt mouse peripheral blood ×3 wt mouse bone marrow ×3 wt mouse lung (wt_pmn_deletion) ×3 pmn deletion mouse lung (wt_pmn_deletion) ×3 wt mouse lung (wt_cko) ×2 cko mouse lung (wt_cko) ×2 wt mouse peripheral blood (wt_cko) ×2 +1 more

Supports a between-group comparison across 23 samples.

How we read this: 9 replicated groups read from 23 sample titles; they account for 23 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM8984433 wt mouse lung 1 wt mouse lung
GSM8984434 wt mouse lung 2 wt mouse lung
GSM8984435 wt mouse lung 3 wt mouse lung
GSM8984436 wt mouse peripheral blood 1 wt mouse peripheral blo…
GSM8984437 wt mouse peripheral blood 2 wt mouse peripheral blo…
GSM8984438 wt mouse peripheral blood 3 wt mouse peripheral blo…
GSM8984439 wt mouse bone marrow 1 wt mouse bone marrow
GSM8984440 wt mouse bone marrow 2 wt mouse bone marrow
GSM8984441 wt mouse bone marrow 3 wt mouse bone marrow
GSM8984442 wt mouse lung (wt_cko) 1 wt mouse lung (wt_cko)
GSM8984443 wt mouse lung (wt_cko) 2 wt mouse lung (wt_cko)
GSM8984444 cko mouse lung (wt_cko) 1 cko mouse lung (wt_cko)
GSM8984445 cko mouse lung (wt_cko) 2 cko mouse lung (wt_cko)
GSM8984446 wt mouse peripheral blood (wt_cko) 1 wt mouse peripheral blo…
GSM8984447 wt mouse peripheral blood (wt_cko) 2 wt mouse peripheral blo…
GSM8984448 cko mouse peripheral blood (wt_cko) 1 cko mouse peripheral bl…
GSM8984449 cko mouse peripheral blood (wt_cko) 2 cko mouse peripheral bl…
GSM8984450 wt mouse lung (wt_pmn_deletion) 1 wt mouse lung (wt_pmn_d…
GSM8984451 wt mouse lung (wt_pmn_deletion) 2 wt mouse lung (wt_pmn_d…
GSM8984452 wt mouse lung (wt_pmn_deletion) 3 wt mouse lung (wt_pmn_d…
GSM8984453 pmn deletion mouse lung (wt_pmn_deletion) 1 pmn deletion mouse lung…
GSM8984454 pmn deletion mouse lung (wt_pmn_deletion) 2 pmn deletion mouse lung…
GSM8984455 pmn deletion mouse lung (wt_pmn_deletion) 3 pmn deletion mouse lung…

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GSE298695 Mechanosensing by endothelial Piezo1 promotes liver regeneration after partial hepatectomy via ATF2-ID1-Wnt2 pathway 16 samples shares piezo1
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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.