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Single Cell Sequencing of Mouse Embryo at Early and Mid Gestation

GSE299135 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2025/07/22 Platform GPL34290
Summary
Maternal adaptations are necessary for pregnancy and proper nurturing of a developing embryo. Murine embryogenesis and pregnancy are intensely studied processes, but historically this knowledge has been acquired by analyzing the role of a single molecule at a time. Single cell RNA-sequencing allows for a snapshot of the of cell states for multiple cell populations that can be compared to understand time-dependent pregnancy adaptations, and the dynamic cellular composition of murine embryogenesis during gestation. Here we present scRNA-seq analyses on murine implantation sites at gestational day (GD) 6.5 and 10.5 and examine the temporal changes that occur from early to mid-gestation. These analyses were conducted on C57/bl6J mice to serve as a resource to the community. We have also made an interactive webpage with these datasets, providing way for anyone to further explore genes and cell types of interest without the need for coding skills. The interactive datasets can be found at (link)
Published in
A single cell transcriptomic resource of murine implantation sites at embryonic days 6.5 and 10.5
Garcia Rivas JF, Duan W, Wilson B et al. · Biology of reproduction 2025 · PMID 40643185 · doi:10.1093/biolre/ioaf155
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Direct links to NCBI, no account and no request form: the whole study as GSE299135_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 8 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1272639 and SRA study SRP590230. Searching any of these in the dataset finder brings you back here.

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