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Heterogeneity and Homogeneity in Treg responses to Interleukin-2 [scATAC-seq2: IL2/JES6-1 timecourse]

GSE299361 Mus musculus (mouse) ChIP-seq / ATAC-seq (genome binding and open chromatin) 1 samples Submitted 2025/11/03 Platform GPL30172
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 1 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Mus musculus (mouse)
Samples
1 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Dynamics and variegation in the Treg response to Interleukin-2Proceedings of the National Academy of Sciences of the United States of America · 2025
Summary (written by the submitters)
We investigated the dynamic effects of IL2 exposure on Treg chromatin accessibility over time by performing scATAC-seq on splenic Tregs isolated from mice at mulitple timepoints (1,2,4,8,16,32,64,128hours) after treatment with IL2/JES6-1 complexes or PBS vehicle control.
Published in
Dynamics and variegation in the Treg response to Interleukin-2
Seddu K, Chowdhary K, Henderson M et al. · Proceedings of the National Academy of Sciences of the United States of America 2025 · PMID 41264258 · doi:10.1073/pnas.2518991122
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE299361_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1273970 and SRA study SRP590919. Searching any of these in the dataset finder brings you back here.

Samples in this study

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