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Single-cell RNA sequencing (sc RNAseq) of white adipose tissue of aged mice

GSE300036 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/11/07 Platform GPL34290
Summary
Aging and obesity are associated with pro-inflammatory changes in adipose tissue. Overlapping mechanisms, such as the infiltration of inflammatory macrophages and T cells into visceral adipose tissue, have been implicated in contributing inflammation. However, a comparative analysis from both states is needed to identify distinct regulatory targets. Here, we performed single-cell RNA sequencing of stromal vascular fractions (SVF) isolated from gonadal white adipose tissue (gWAT) of young mice fed a normal or a high-fat diet, and aged mice fed a normal diet. Our analysis revealed that physiological aging, more so than high-fat diet induced obesity, was associated with accumulation of phenotypically distinct CD8 T cells resembling virtual memory (VM) CD8 T cells.
Published in
Single-Cell RNA Sequencing Identifies Accumulation of Fcgr2b + Virtual Memory-Like CD8 T Cells With Cytotoxic and Inflammatory Potential in Aged Mouse White Adipose Tissue
Kumar A, O'Brien M, Young VB et al. · Aging cell 2025 · PMID 41116748 · doi:10.1111/acel.70278
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Also filed as BioProject PRJNA1278734 and SRA study SRP592649. Searching any of these in the dataset finder brings you back here.

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