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Single-cell transcriptome profiling of tracheal epithelium in basal cell-specific Mettl3 conditional knockout mice

GSE300742 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2026/06/25 Platform GPL24247
Summary
The airway epithelium plays critical roles in respiratory function and is implicated in various pulmonary diseases. To investigate the role of the RNA methyltransferase Mettl3 in airway basal cell homeostasis, we generated a comprehensive single-cell RNA sequencing atlas of tracheal epithelial cells from Mettl3 conditional knockout (M3-CKO) mice and littermate controls. Our dataset specifically focuses on Krt5-lineage traced basal cells, comparing tdTomato+ (traced) and tdTomato- (non-traced) populations from both genotypes.We present a comprehensive single-cell RNA-sequencing atlas of tracheas, comprising diverse populations of cells, epithelial cell, myeloid cells, B lymphocytes, T lymphocytes, endothelial cells, fibroblasts and glandular mucous cells.
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Direct links to NCBI, no account and no request form: the whole study as GSE300742_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1282005 and SRA study SRP594517. Searching any of these in the dataset finder brings you back here.

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