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Single-cell RNA-seq analysis of cervical lymph nodes from mice intradermally infected with Mycobacterium tuberculosis

GSE300819 Mus musculus Expression profiling by high throughput sequencing 6 samples Submitted 2025/11/20 Platform GPL21273
Summary
In mice, establishment of a contained and persistent yet non-pathogenic infection with Mtb ("contained Mtb infection", CMTB) rapidly and durably reduces tuberculosis disease burden after re-exposure through aerosol challenge. The bacteria are introduced by intradermal inoculation into the ear and are contained in the corresponsing cervical lymph node. Protection is associated with elevated activation of alveolar macrophages, the first cells that respond to inhaled Mtb, and accelerated recruitment of Mtb-specific T cells to the lung parenchyma. This study used single-cell RNA-seq to compare the cellularity and transcriptional profiles of innate immune cells in the cervical lymph node of naive mice and mice with CMTB.
Published in
c-Myc Inhibits Macrophage Antimycobacterial Response in Mycobacterium tuberculosis Infection
Sarti E, Dollé C, Wolfensberger R et al. · The Journal of infectious diseases 2025 · PMID 40884499 · doi:10.1093/infdis/jiaf456
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Also filed as BioProject PRJNA1282144 and SRA study SRP594684. Searching any of these in the dataset finder brings you back here.

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