← BioTransfer GEO Dataset Finder
GEO series

Serum Response Factor (SRF) drives actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity

GSE300990 Mus musculus (mouse) ChIP-seq / ATAC-seq (genome binding and open chromatin) 30 samples Submitted 2026/02/16 Platform GPL19057Platform GPL24247
At a glance

ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 30 samples

What it is
ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
Organism
Mus musculus (mouse)
Samples
30 samples: 6 × SRF floxed WT, bulk RNA-seq, eWAT, vs 6 × SRF-AKO, bulk RNA-seq, eWAT, vs 6 × SRF floxed WT, bulk RNA-seq, iWAT, vs 6 × SRF-AKO, bulk RNA-seq, iWAT,Groups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in miceMetabolism: clinical and experimental · 2026
Summary (written by the submitters)
Background: Adipocyte hypertrophy, the unique ability of adipocytes to expand in response to positive energy balance, is a key determinant of metabolic health during obesity. However, the molecular mechanisms that govern this adaptive growth are not fully understood. Methods: We performed super-enhancer profiling to identify transcriptional regulators activated in adipocytes under obesogenic conditions.
Read the full summary ↓
Functional roles of the identified regulator were assessed through in vitro and in vivo analyses, including adipocyte-specific deletion of target gene and subsequent single-nucleus RNA sequencing. Results: Serum Response Factor (SRF) was identified as a pivotal driver of actin cytoskeletal remodeling in adipocytes during obesity. SRF was both necessary and sufficient for the expression of actin cytoskeletal genes in vitro. In vivo, adipocyte-specific SRF deletion reduced actin cytoskeletal gene expression, disrupted filamentous actin networks, and impaired adipocyte enlargement following high-fat diet feeding. Despite similar body weights, mice lacking adipocyte SRF exhibited exacerbated insulin resistance and ectopic lipid accumulation in the liver and brown adipose tissues, reflecting compromised adipocyte integrity and lipid storage capacity. Single-nucleus RNA-seq further revealed that defective actin cytoskeletons in adipocytes disrupted tissue homeostasis, resulting in impaired vascularization and elevated inflammation. Conclusion: These findings establish SRF as a central regulator of actin cytoskeletal organization in adipocytes, essential for supporting healthy hypertrophic expansion and proper tissue remodeling during obesity. SRF emerges as a critical determinant of metabolic health and a potential therapeutic target for obesity-related disorders.
Published in
Serum Response Factor (SRF) promotes actin cytoskeletal organization in adipocytes to support adaptive hypertrophic expansion and tissue remodeling during obesity in mice
So J, Wann J, Kim K et al. · Metabolism: clinical and experimental 2026 · PMID 41655957 · doi:10.1016/j.metabol.2026.156548
This dataset
Open GSE300990 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE300990_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.

Also filed as BioProject PRJNA1282965 and SRA study SRP595561. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41655957) ↗
Study design
6 × SRF floxed WT, bulk RNA-seq, eWAT, vs 6 × SRF-AKO, bulk RNA-seq, eWAT, vs 6 × SRF floxed WT, bulk RNA-seq, iWAT, vs 6 × SRF-AKO, bulk RNA-seq, iWAT,

Supports a case/control comparison: 1 samples read as cases, 15 as controls.

How we read this: 4 replicated groups read from 30 sample titles; they account for 24 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.