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RNA-seq of primary cardiomyocytes under ischemia-reperfusion injury reveals altered expression in DNA damage repair genes including Xrcc6

GSE301051 Mus musculus Expression profiling by high throughput sequencing 7 samples Submitted 2025/09/04 Platform GPL24247
Summary
To investigate the molecular response of cardiomyocytes to ischemia-reperfusion (I/R) injury, we established an in vitro I/R model using primary neonatal mouse cardiomyocytes. Oxidative stress was simulated by treating the cells with 100 μM hydrogen peroxide (H₂O₂) for 24 hours, followed by a 24-hour recovery period in complete medium to mimic the ischemia and reperfusion phases, respectively. Bulk RNA sequencing was performed on both control (n = 3) and I/R-treated (n = 4) cardiomyocytes. Transcriptomic profiling identified differentially expressed genes and enriched pathways associated with oxidative stress response, DNA damage repair, and regulation of cell death. This dataset provides valuable insights into the molecular mechanisms underlying myocardial I/R injury and may help identify potential therapeutic targets.
Published in
Xrcc6 coordinates cardiomyocyte repair and immune regulation in myocardial ischemia-reperfusion injury: Fisetin as a therapeutic modulator
He Y, Li J, Guo L et al. · Frontiers in immunology 2025 · PMID 41041340 · doi:10.3389/fimmu.2025.1653738
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Direct links to NCBI, no account and no request form: the whole study as GSE301051_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 7 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1282757 and SRA study SRP594939. Searching any of these in the dataset finder brings you back here.

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