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KRT6A enhances radiotherapy resistance in lung squamous cell carcinoma via activation of the autophagy pathway through RAB39B [RNA-Seq 2]

GSE301226 Homo sapiens Expression profiling by high throughput sequencing 4 samples Submitted 2026/06/29 Platform GPL34284
Summary
Abstract Background: Radiotherapy is the cornerstone for lung squamous cell carcinoma (LUSC) treatment, yet its efficacy is often limited by the development of radioresistance. Understanding the molecular mechanisms driving this resistance is critical for improving therapeutic outcomes. Results: In this study, we established a radioresistant LUSC cell line and identified KRT6A as a pivotal gene involved in radiotherapy resistance. Transcriptome sequencing and further validation revealed RAB39B as a downstream target of KRT6A. The KRT6A-RAB39B axis promotes radioresistance by activating the autophagy pathway, enhancing cancer cell survival in response to radiation stress. Mechanistic studies confirmed that KRT6A regulates autophagy through modulation of RAB39B expression. Conclusions:This work is the first to uncover a role of the KRT6A-RAB39B axis in LUSC radioresistance, offering new insights into the mechanisms and potential targets underlying radiotherapy failure.
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Direct links to NCBI, no account and no request form: the whole study as GSE301226_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1284298 and SRA study SRP596252. Searching any of these in the dataset finder brings you back here.

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