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RNA-seq analysis of arterial smooth muscle cells (ASMCs) isolated from the lung, heart, aorta, stomach, intestine, kidney and bladder

GSE301477 Mus musculus (mouse) RNA-seq (gene expression) 22 samples Submitted 2026/04/11 Platform GPL19057
At a glance

RNA-seq (gene expression) · mouse · 22 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
22 samples: 7 conditions, mostly in triplicateGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Cell Type-Specific Targeting of Different Smooth Muscle Cell Populations by Intersectional GeneticsCirculation · 2026
Summary (written by the submitters)
None of the currently available Cre recombinase-expressing mouse lines allows exclusive and specific manipulation of arterial smooth muscle cells (ASMCs). To enable targeted manipulation of ASMCs, we generated two knock-in mouse lines: one carrying a Dre recombinase cassette inserted into the Cspg4 locus (Cspg4-Dre) and another carrying a Rox-Stop-Rox-CreER cassette inserted into the Acta2 locus (Acta2-Rox-CreER).
Read the full summary ↓
These two lines (hereafter referred to as ASMC-effector mice) were crossed with R26-LoxP-GFP reporter animals, and the offspring were treated with three doses of tamoxifen to induce recombination. This strategy resulted in robust and specific labeling of ASMCs in virtually all arteries examined.To further enhance specificity, we generated an additional line in which a tamoxifen-inducible DreERT cassette was inserted into the Cspg4 locus. This Cspg4-DreERT line also enabled specific labeling of ASMCs in distinct organs.
Published in
Cell Type-Specific Targeting of Different Smooth Muscle Cell Populations by Intersectional Genetics
Wang L, Staps M, Günther S et al. · Circulation 2026 · PMID 41969103 · doi:10.1161/CIRCULATIONAHA.125.076318
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE301477_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1285306 and SRA study SRP597294. Searching any of these in the dataset finder brings you back here.

Study design
7 conditions, mostly in triplicate
Intestine ×4 Aorta ×3 Bladde ×3 Kidney ×3 Lung ×3 Stomach ×3 Heart ×3

Supports a between-group comparison across 22 samples.

How we read this: 7 replicated groups read from 22 sample titles; they account for 22 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
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