RNA-seq analysis of pulmonary arterial smooth muscle cells (PASMCs) and bronchial smooth muscle cells (BSMCs) after pulmonary arterial hypertension (PAH) treatment
RNA-seq (gene expression) · mouse · 12 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 12 samples: 3 × PASMCs-hox vs 3 × PASMCs-nox vs 3 × BSMCs-hox vs 3 × BSMCs-noxGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Cell Type-Specific Targeting of Different Smooth Muscle Cell Populations by Intersectional GeneticsCirculation · 2026
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE301479_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1285310 and SRA study SRP597322. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 12 samples.
How we read this: 4 replicated groups read from 12 sample titles; they account for 12 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM9083860 PASMCs-hox_1 PASMCs-hox
- GSM9083861 PASMCs-hox_2 PASMCs-hox
- GSM9083862 PASMCs-hox_3 PASMCs-hox
- GSM9083863 PASMCs-nox_1 PASMCs-nox
- GSM9083864 PASMCs-nox_2 PASMCs-nox
- GSM9083865 PASMCs-nox_3 PASMCs-nox
- GSM9083866 BSMCs-hox_1 BSMCs-hox
- GSM9083867 BSMCs-hox_2 BSMCs-hox
- GSM9083868 BSMCs-hox_3 BSMCs-hox
- GSM9083869 BSMCs-nox_1 BSMCs-nox
- GSM9083870 BSMCs-nox_2 BSMCs-nox
- GSM9083871 BSMCs-nox_3 BSMCs-nox
- GSE301478 RNA-seq analysis of pulmonary arterial smooth muscle cells (PASMCs), bronchial smooth muscle cells(BSMCs) and venous SMCs in the lung 9 samples shares pulmonary, bronchial, arterial, rna-seq, smooth
- GSE255061 Phosphatase-independent activity of smooth-muscle calcineurin orchestrates a gene expression program leading to arterial hypertension 40 samples shares hypertension, arterial, smooth
- GSE316502 Transcriptomic Profiling of the Sex-linked Biological Pathways of Severe Pulmonary Arterial Hypertension associated with Endothelial Cell Caveolin-1 Depletion and Chronic Hypoxia 35 samples shares hypertension, pulmonary, arterial
- GSE305242 Effects of Sphk1 deletion in smooth muscle cells on murine arterial transcriptome in hypertension 26 samples shares hypertension, arterial, smooth
- GSE315475 Mice with targeted deletion of the Vasoactive Intestinal Peptide (VIP) gene have spontaneous pulmonary arterial hypertension exaggerated with hypoxia 20 samples shares hypertension, pulmonary, arterial
- GSE316584 MicroRNA-224 Orchestrates the BMP Pathway and Represents a New Therapeutic Target for Pulmonary Arterial Hypertension 9 samples shares hypertension, pulmonary, arterial
- GSE295426 Targeting branched-chain amino acids alleviates pulmonary fibrosis [RNA-seq] 54 samples shares pulmonary, rna-seq
- GSE287948 Let-7 restrains an oncogenic epigenetic circuit in AT2 cells to prevent ectopic formation of fibrogenic cell intermediates and pulmonary fibrosis [RNA-seq] 24 samples shares pulmonary, rna-seq
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.