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Effects of Sphk1 deletion in smooth muscle cells on murine arterial transcriptome in hypertension

GSE305242 Mus musculus (mouse) RNA-seq (gene expression) 26 samples Submitted 2026/04/07 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 26 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
26 samples: 6 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Vascular Sphingosine Kinase 1 Regulates Angiotensin II-Induced HypertensionHypertension (Dallas, Tex. : 1979) · 2026
Summary (written by the submitters)
The function of the cardiovascular system is regulated by sphingosine-1-phosphate (S1P) signalling. Vascular sphingosine kinase 1 (Sphk1), an S1P-producing enzyme, is upregulated in hypertension (HTN), and mice lacking Sphk1 globally exhibit alleviated angiotensin II (AngII)-induced HTN. Identifying the cellular mechanisms that provide this protection remains challenging due to the pleiotropic action of S1P.
Read the full summary ↓
To obtain mice lacking Sphk1 in smooth muscle cells (SMCs), Sphk1 f/f (flox/flox) mice were crossed with transgenic mice harboring Cre recombinase gene under control of the mouse smooth muscle protein 22-alpha/transgelin (SM22α/Tagln) promoter. Subsequently, resulting Sphk1f/-Tagln-cre+ offsprings were mated to Sphk1f/f mice to generate mice lacking Sphk1 specifically in SMCs (Sphk1f/fTagln-cre+, later referred to as SMC KO-Sphk1) and control mice (Sphk1f/fTagln-cre-, hereafter referred to as wild type (WTf/f) mice).
Published in
Vascular Sphingosine Kinase 1 Regulates Angiotensin II-Induced Hypertension
Jozefczuk E, Szczepaniak P, Mikolajczyk TP et al. · Hypertension (Dallas, Tex. : 1979) 2026 · PMID 41944022 · doi:10.1161/HYPERTENSIONAHA.125.25480
This dataset
Open GSE305242 in finder → Find more mouse RNA-seq datasets →
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓
2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE305242_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓
3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1304912 and SRA study SRP608392. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41944022) ↗
Study design
6 conditions, mostly with about 4 replicates each
Aorta, hypertensive Sphk1 SMC KO ×5 Mesenteric arteries, hypertensive Sphk1 SMC KO ×5 Aorta, hypertensive Sphk1 flox/flox ×4 Aorta, normotensive Sphk1 flox/flox ×4 Mesenteric arteries, hypertensive Sphk1 flox/… ×4 Mesenteric arteries, normotensive Sphk1 flox/… ×4

Supports a between-group comparison across 26 samples.

How we read this: 6 replicated groups read from 26 sample titles; they account for 26 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study
Datasets on a similar topic

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