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Gene expression profile at single nucleus level of hippocampal cells from APP-TG mice treated with Low-dose ∆9-THC and Celecoxib

GSE301659 Mus musculus Expression profiling by high throughput sequencing 5 samples Submitted 2025/12/04 Platform GPL24247
Summary
Delta-9-tetrahydrocannabinol (Δ9-THC) is reported to rescue cognitive and synaptic functions in Alzheimer's disease (AD) mice model. However, Δ9-THC treatment resulted in neuroinflammation via activating Cox2 in the brain. Our hypothesis is that inhibiting cox2 via celecoxib treatment could inhibit neuroinflammation and enhance the neuroprotective effects of Δ9-THC. Our results indicated that Cel-THC treatment inhibited spatial learning and memory impairments of APP-TG mice via water maze test. To investigate the molecular mechanisms, we performed single nucleus RNA sequence of hippocampal cells from WT-Veh, APP-TG-Veh, APP-TG-THC, APP-TG-Cel, and APP-TG-Cel-THC groups. Our results showed that Cel-THC treatment reversed changes of synaptic, inflammation-, and AD-related differentially expressed genes caused by beta amyloid accumulation.
Published in
A Combination of Low-Dose Δ(9)-THC and Celecoxib as a Therapeutic Strategy for Alzheimer's Disease
Zhang J, Zhu D, Hu M et al. · Aging and disease 2025 · PMID 41452714 · doi:10.14336/AD.2025.1206
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Also filed as BioProject PRJNA1285933 and SRA study SRP598135. Searching any of these in the dataset finder brings you back here.

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